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Gene Expression Data
Assay Details
Assay
Reference: J:260417 Miesfeld JB, et al., The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody. Gene Expr Patterns. 2018 Jan;27:114-121
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6195177
Gene symbol: Atoh7
Gene name: atonal bHLH transcription factor 7
Antibody: ATOH7 Antibody (NBP1-88639)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 2C: embryonic day 13.5; Atoh7tm1Gla/Atoh7+ (more )
Note: Double labeled: purple - Atoh7 protein; green - Atoh7 reporter (assay MGI:6195191).
Structure Level Pattern Image Note
TS21: retina Present Punctate 2C right , 2C left In a salt and pepper pattern.
Specimen 2D: embryonic day 13.5; Atoh7tm1Gla/Atoh7tm1Gla (more )
Note: Double labeled: purple - Atoh7 protein; green - Atoh7 reporter (assay MGI:6195191).
Structure Level Pattern Image Note
TS21: retina Absent 2D right , 2D left
Specimen 3A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: retina Present Regionally restricted 3A lower , 3A upper Expressed in the central retina.
Specimen 3B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: retina Present Regionally restricted 3B lower , 3B upper Expression expanded toward the periphery.
Specimen 3C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: retina Present 3C upper , 3C lower Expression expanded toward the periphery and reached the edge of the optic cup.
Specimen 3D upper: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina Present Regionally restricted 3D upper Expressed sparsely in the proliferative layer of the central retina.
Specimen 3D lower: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina Moderate 3D lower
Specimen 4A: embryonic day 13.5 (more )
Note: Double labeled: purple - Atoh7; green - Neurog2 (assay MGI:6195246).
Structure Level Pattern Image Note
TS21: retina Present 4A high left , 4A , 4A high right Neurog2 and Atoh7 double positive cells were detected in the neuroblast layer, but not in differentiated retinal ganglion cells.
Specimen 4B: embryonic day 13.5 (more )
Note: Double labeled: purple - Atoh7; green - Pou4f1 (assay MGI:6195247).
Structure Level Pattern Image Note
TS21: retina Present 4B high left , 4B high right , 4B Pou4f1 was transiently expressed with Atoh7 during retinal ganglion cells migration and maintained in the ganglion cell layer.
Specimen 4C: embryonic day 13.5 (more )
Note: Double labeled: purple - Atoh7; green - Isl1 (assay MGI:6195299).
Structure Level Pattern Image Note
TS21: retina Present 4C high left , 4C high right , 4C Isl1 was transiently expressed with Atoh7 during retinal ganglion cells migration and maintained in the ganglion cell layer.
Specimen 4D: embryonic day 13.5 (more )
Note: Double labeled: purple - Atoh7; green - Tubb3 (assay MGI:6195248).
Structure Level Pattern Image Note
TS21: retina Present 4D high left , 4D high right , 4D Tubb3 was coexpressed with Atoh7 in some migrating cells apical to the ganglion cell layer.
Specimen 5A: embryonic day 13.5; Atoh7tm1.1Xmu/Atoh7+ (more )
Note: Double labeled: purple - Atoh7 protein; green - Atoh7 reporter (assay MGI:6195250).
Structure Level Pattern Image Note
TS21: retina Present 5A high right , 5A , 5A high left The HA knock-in completely overlapped endogenous Atoh7.
Specimen 5C: embryonic day 13.5; Atoh7tm1Gla/Atoh7+ (more )
Note: Double labeled: purple - Atoh7 protein; green - Atoh7 reporter (assay MGI:6195191).
Structure Level Pattern Image Note
TS21: retina Present 5C high right , 5C high left , 5C Atoh7+lacZ-cells (youngest) are restricted to the neuroblast layer, whereas Atoh7-lacZ+ cells (oldest) represent differentiating neurons, e.g. retinal ganglion cells in the ganglion cell layer and cones at the apex. Atoh7+lacZ+ cells (intermediate stage) were mainly located in the neuroblast layer.
Specimen 6A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: retina Present 6A Expressed in dividing progenitors.
Specimen 6B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: retina Present 6B Expressed in dividing progenitors.
Specimen S1A: embryonic day 13.5; Atoh7tm1.1Xmu/Atoh7+ (more )
Structure Level Pattern Image Note
TS21: retina Present S1A
Specimen not shown: embryonic day 13.5; Atoh7tm1.1Xmu/Atoh7tm1.1Xmu (more )
Structure Level Pattern Image Note
TS21: retina Present Similar to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory