Atoh7^tm1.1Xmu
Targeted Allele Detail

Summary

• Symbol: Atoh7^tm1.1Xmu
• Name: atonal bHLH transcription factor 7; targeted mutation 1.1, Xiuqian Mu
• MGI ID: MGI:4353889
• Synonyms: Math5^HA
• Gene: Atoh7 Location: Chr10:62935564-62937059 bp, + strand Genetic Position: Chr10, 32.54 cM
• Alliance: Atoh7^tm1.1Xmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151244
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Sequence encoding three copies of the hemagglutinin (HA) epitope YPYDVPDYA were fused in frame 3' to the single exon of the gene locus. A floxed neomycin cassette was inserted downstream of the exon and was later removed by breeding founders with a cre deleter strain. Mice homozygous for this C-terminal "tagged" allele recapitulated wild-type expression patterns of the endogenous protein. Protein function was unaltered as determined by phenotypic analysis. (J:151244)

Expression

In Mice Carrying this Mutation:

38 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atoh7 Mutation: 9 strains or lines available

References

• Original: J:151244 Fu X, et al., Epitope-tagging Math5 and Pou4f2: New tools to study retinal ganglion cell development in the mouse. Dev Dyn. 2009 May 20;238(9):2309-2317
• All: 9 reference(s)