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Gene Expression Data
Assay Details
Assay
Reference: J:260417 Miesfeld JB, et al., The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody. Gene Expr Patterns. 2018 Jan;27:114-121
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6195191
Gene symbol: Atoh7
Gene name: atonal bHLH transcription factor 7
Reporter: lacZ
Antibody: anti-B-gal
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2C: embryonic day 13.5; Atoh7tm1Gla/Atoh7+ (more )
Note: Double labeled: green - Atoh7 reporter; purple - Atoh7 protein (assay MGI:6195177).
Structure Level Pattern Image Note
TS21: retina Present Punctate 2C right , 2C middle In a salt and pepper pattern.
Specimen 2D: embryonic day 13.5; Atoh7tm1Gla/Atoh7tm1Gla (more )
Note: Double labeled: green - Atoh7 reporter; purple - Atoh7 protein (assay MGI:6195177).
Structure Level Pattern Image Note
TS21: retina Present Punctate 2D right , 2D middle In a salt and pepper pattern. Stronger expression than in the heterozygous.
Specimen 5C: embryonic day 13.5; Atoh7tm1Gla/Atoh7+ (more )
Note: Double labeled: green - Atoh7 reporter; purple - Atoh7 protein (assay MGI:6195177).
Structure Level Pattern Image Note
TS21: retina Present Punctate 5C high right , 5C , 5C high middle Atoh7+lacZ-cells (youngest) are restricted to the neuroblast layer, whereas Atoh7-lacZ+ cells (oldest) represent differentiating neurons, e.g. retinal ganglion cells in the ganglion cell layer and cones at the apex. Atoh7+lacZ+ cells (intermediate stage) were mainly located in the neuroblast layer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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