Piezo2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:211312 Woo SH, et al., Piezo2 is required for Merkel-cell mechanotransduction. Nature. 2014 May 29;509(7502):622-6
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5697547
Gene symbol:	Piezo2
Gene name:	piezo-type mechanosensitive ion channel component 2

Antibody:	anti-Piezo2
Detection system:	Secondary antibody

Results

Specimen Ext. 1C: postnatal adult; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Double labeled: green - Piezo2 protein; red - Piezo2 reporter (assay MGI:5697408).

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Non-Uniform	Extended Data 1C Merge , Extended Data 1C Piezo2	Expression is seen in 45.6% of the neurons; high expression levels are seen in a quarter of those neurons.

Specimen Ext. 1D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Non-Uniform	Extended Data 1D Piezo2 , Extended Data 1D Merge	Expression is seen in 45.6% of the neurons; high expression levels are seen in a quarter of those neurons.

Specimen Ext. 3C: postnatal newborn (more

)

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		Extended Data 3C

Specimen Ext. 3D: postnatal newborn; Piezo2^tm2.1Apat/Piezo2^tm2.1Apat (more

)

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Absent		Extended Data 3D

Specimen 2D: postnatal; Piezo2^tm2.2Apat/Piezo2^tm2.2Apat (more

)
Note: Whisker pad. Double labeled: green - Piezo2; red - Krt8 (assay MGI:5697549).

Structure	Level	Pattern	Image	Note
TS28: face skin	Present	Single cells	2D Piezo2 , 2D Krt8/Piezo2	Expression is seen in the Merkel cells of the whisker pads.

Specimen 2E: postnatal; Piezo2^tm2.2Apat/Piezo2^tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Whisker pad. Double labeled: green - Piezo2; red - Krt8 (assay MGI:5697549).

Structure	Level	Pattern	Image	Note
TS28: face skin	Absent		2E Piezo2 , 2E Krt8/Piezo2	In the mutant, expression has been abolished from all epithelial cells, including Merkel cells.

Specimen 2F: postnatal; Piezo2^tm2.2Apat/Piezo2^tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Whisker pad. Triple labeled: green - Piezo2; red - Krt8 (assay MGI:5697549); blue - Nefh (assay MGI:5697548).

Structure	Level	Pattern	Image	Note
TS28: face skin	Absent		2F Krt8/Piezo2/Nefh , 2F Piezo2	In the mutant, expression has been abolished from all epithelial cells, including Merkel cells.
TS28: peripheral nervous system	Present	Regionally restricted	2F Piezo2 , 2F Krt8/Piezo2/Nefh	Expression is present in the sensory afferents that innervate Merkel cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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