Piezo2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:211312 Woo SH, et al., Piezo2 is required for Merkel-cell mechanotransduction. Nature. 2014 May 29;509(7502):622-6
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5697408
Gene symbol:	Piezo2
Gene name:	piezo-type mechanosensitive ion channel component 2

Results

Specimen Ext. 1C: postnatal adult; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Double labeled: red - Piezo2 reporter; green - Piezo2 protein (assay MGI:5697547).

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Non-Uniform	Extended Data 1C GFP , Extended Data 1C Merge	Expression is seen in 45.6% of the neurons; high expression levels are seen in a quarter of those neurons.

Specimen Ext. 2D: postnatal; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Whisker pad. Double labeled: green - Piezo2 reporter; red - Nefh (assay MGI:5697548).

Structure	Level	Pattern	Image	Note
TS28: face skin	Present	Single cells	Extended Data 2E , Extended Data 2D , Extended Data 2H	Expression is seen in Merkel cells in the whisker pad and the afferent fibers.

Specimen 1B: postnatal; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Whisker pad. Double labeled: green - Piezo2 reporter; red - Krt8 (assay MGI:5697549).

Structure	Level	Pattern	Image	Note
TS28: face skin	Present	Single cells	1B GFP , 1B GFP/Krt8	Expression is seen in Merkel cells in the whisker pad.

Specimen 1C/D: postnatal; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Whisker pad. Triple labeled: green - Piezo2 reporter; red - Krt8 (assay MGI:5697549); blue - Nefh (assay MGI:5697548).

Structure	Level	Pattern	Image	Note
TS28: face skin	Present	Single cells	1C GFP , 1D GFP , 1D GFP/Krt8/Nefh , 1C GFP/Krt8/Nefh	Expression is seen in Merkel cells in the whisker pad and the afferent fibers.
TS28: peripheral nervous system	Present	Single cells	1C GFP , 1D GFP , 1D GFP/Krt8/Nefh , 1C GFP/Krt8/Nefh	Expression is seen in Merkel cells in the whisker pad and the afferent fibers.

Specimen 1E: postnatal; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Dorsal skin. Double labeled: green - Piezo2 reporter; red - Krt8 (assay MGI:5697549).

Structure	Level	Pattern	Image	Note
TS28: back skin	Present		1E GFP/Krt8 , 1E GFP	Expression is seen in Merkel cells in touch domes in hairy skin.

Specimen 1F: postnatal; Piezo2^{tm1.1(cre)Apat}/Piezo2^{tm1.1(cre)Apat} (more

)
Note: Foot pad. Double labeled: green - Piezo2 reporter; red - Krt8 (assay MGI:5697549).

Structure	Level	Pattern	Image	Note
TS28: autopod skin	Present		1F GFP , 1F GFP/Krt8	Expression is seen in Merkel cells in glabrous (nonhairy) skin.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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