Piezo2^tm2.1Apat
Targeted Allele Detail

Summary

• Symbol: Piezo2^tm2.1Apat
• Name: piezo-type mechanosensitive ion channel component 2; targeted mutation 2.1, Ardem Patapoutian
• MGI ID: MGI:5574480
• Synonyms: Piezo2^-
• Gene: Piezo2 Location: Chr18:63143284-63520254 bp, - strand Genetic Position: Chr18, 35.95 cM, cytoband D3
• Alliance: Piezo2^tm2.1Apat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211312
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was constructed to insert loxP sites upstream of exon 43 and downstream of exon 45 of the Piezo2 gene. The vector contained an frt-flanked PGK-neo cassette upstream of the first loxP site. Following homologous recombination in ES cells, the PGK-neo marker and a single loxP site were upstream of exon 43 and a second loxP site was downstream of exon 45.Chimeric animals were crossed to EIIa-cre expressing mice to delete exons 43-45, causing a frameshift that results in production of a premature stop codon. (J:211312)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piezo2 Mutation: 123 strains or lines available

References

• Original: J:211312 Woo SH, et al., Piezo2 is required for Merkel-cell mechanotransduction. Nature. 2014 May 29;509(7502):622-6
• All: 3 reference(s)