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Col1a1tm1(tetO-Yod1*)Hpl
Targeted Allele Detail
Summary
Symbol: Col1a1tm1(tetO-Yod1*)Hpl
Name: collagen, type I, alpha 1; targeted mutation 1, Hidde Ploegh
MGI ID: MGI:5478606
Synonyms: YOD1-C160S
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm1(tetO-Yod1*)Hpl page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:194608
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Col1a1tm1(tetO-Yod1*)Hpl expresses 1 gene
 
Mutation detailsRMCE on KH2 cells inserted a cassette into the 3' UTR. The cassette contains the tetracycline responsive element (tetO) driving inducible expression of a C-terminal HA-tagged Yod1) with nucleotide substitutions that result in the amino acid substitution of serine for cysteine at position 160 (C160S). This mutation is expected to produce a catalytically inactivated product. (J:194608)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  160 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)(J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:194608 Sehrawat S, et al., A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation. Blood. 2013 Feb 14;121(7):1145-56
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory