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Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5461570
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Reporter: lacZ
Antibody: anti-B-galactosidase (Cappel/ICN/MP Biomedicals)
Detection system: Secondary antibody coupled to Cy2
Assay notes: The authors described using three antibodies but did not specify which was used in the pictured assay; it is possible either MGI:3575669 or MGI:3529591 was used to generate some or all of these data.
Results
Specimen 3A: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: blue - Pitx2 reporter; green - Myog (assay MGI:5461568); red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS15: trunk dermomyotome Absent 3A
TS15: trunk myotome Absent 3A
Specimen 3A': embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: blue - Pitx2 reporter; green - Myog (assay MGI:5461568); red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS15: mesenchyme derived from somatopleure Present Regionally restricted 3A' Expression is limited to the ventral somatopleure.
Specimen 3B: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: blue - Pitx2 reporter; green - Myog (assay MGI:5461568); red - Lbx1 (assay MGI:5461566).
Structure Level Pattern Image Note
TS15: trunk dermomyotome Absent 3B
TS15: trunk myotome Absent 3B
Specimen 3B': embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: blue - Pitx2 reporter; green - Myog (assay MGI:5461568); red - Lbx1 (assay MGI:5461566).
Structure Level Pattern Image Note
TS15: mesenchyme derived from somatopleure Present Regionally restricted 3B' Expression is limited to the ventral somatopleure.
Specimen Not shown: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Structure Level Pattern Image Note
TS15: 1st branchial arch Present
Specimen 4A: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: blue - Pitx2 reporter; green - Pax3 (assay MGI:5461523); red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: tongue muscle Present 4A
TS20: head mesenchyme Present Regionally restricted 4A Expression is present in the masseter anlagen.
TS20: maxillary process mesenchyme Absent 4A There is no expression in neural crest cells in the maxilla component.
Specimen 4B: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Triple labeled: blue - Pitx2 reporter; green - Lbx1 (assay MGI:5461565); red - myosin.
Structure Level Pattern Image Note
TS20: tongue muscle Present 4B
TS20: head mesenchyme Present Regionally restricted 4B Expression is present in the masseter anlagen.
Specimen 4C: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: blue - Pitx2 reporter; green - Pax3 (assay MGI:5461523); red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: paraxial mesenchyme Present Regionally restricted 4C Expression is present in the deep back muscle anlagen, intercostals muscles, latissimus dorsi and abdominal muscle anlagen.
TS20: limb mesenchyme Present Regionally restricted 4C Expression is present in the extensor and flexor muscle anlagen.
Specimen 4D: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Triple labeled: blue - Pitx2 reporter; green - Lbx1 (assay MGI:5461565); red - myosin.
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 4D Expression is present in the biceps branchii and extensor and flexor muscle anlagen.
TS20: paraxial mesenchyme Present Regionally restricted 4D Expression is present in the latissimus dorsi and intercostals anlagen.
TS20: body wall mesenchyme Present 4D Expression is present in the ventral somatopleure.
TS20: embryo mesenchyme Present Regionally restricted 4D Expression is present in the dorsal somatopleure.
Specimen 4E: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: blue - Pitx2 reporter; green - Pax3 (assay MGI:5461523); red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 4E Expression is present in the muscle anlagen.
Specimen 4F: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Triple labeled: blue - Pitx2 reporter; green - Lbx1 (assay MGI:5461565); red - myosin.
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 4F Expression is present in the muscle anlagen, including flexors and extensors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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