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Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5461568
Gene symbol: Myog
Gene name: myogenin
Antibody: Anti-Myogenin (M-225; sc-576)
Detection system: Secondary antibody coupled to Cy3
Assay notes: The authors described using two antibodies but did not specify which was used in the pictured assay; it is possible MGI:5461327 was used to generate some or all of these data.
Results
Specimen 3A: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: green - Myog; blue - Pitx2 reporter (assay MGI:5461570); red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS15: trunk myotome Present 3A
Specimen 3A': embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: green - Myog; blue - Pitx2 reporter (assay MGI:5461570); red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS15: mesenchyme derived from somatopleure Absent 3A' There is no expression in the ventral somatopleure.
Specimen 3B: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: green - Myog; blue - Pitx2 reporter (assay MGI:5461570); red - Lbx1 (assay MGI:5461566).
Structure Level Pattern Image Note
TS15: trunk myotome Present 3B
Specimen 3B': embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: green - Myog; blue - Pitx2 reporter (assay MGI:5461570); red - Lbx1 (assay MGI:5461566).
Structure Level Pattern Image Note
TS15: mesenchyme derived from somatopleure Absent 3B' There is no expression in the ventral somatopleure.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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