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Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5461520
Gene symbol: Pax3
Gene name: paired box 3
Antibody: rat anti-Pax3
Detection system: Secondary antibody coupled to Cy5
Results
Specimen 3A: embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: red - Pax3; green - Myog (assay MGI:5461568); blue - Pitx2 reporter (assay MGI:5461570).
Structure Level Pattern Image Note
TS15: trunk dermomyotome Present 3A
Specimen 3A': embryonic day 9.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Triple labeled: red - Pax3; green - Myog (assay MGI:5461568); blue - Pitx2 reporter (assay MGI:5461570).
Structure Level Pattern Image Note
TS15: mesenchyme derived from somatopleure Absent 3A' There is no expression in the ventral somatopleure.
Specimen 3C: embryonic day 10.0; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level. Double labeled: red - Pax3; green - Pitx2 protein (assay MGI:5461567).
Structure Level Pattern Image Note
TS16: forelimb bud mesenchyme Present 3C Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
Specimen 3C upper: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: This is a taken at the mid forelimb level.
Structure Level Pattern Image Note
TS16: gut Absent 3C upper
Specimen 3D: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3D Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
Specimen 3F: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS17: trunk myotome Present 3F Expression is present in the migrating limb muscle precursors; some are shown at higher magnification in the inset.
Specimen 3G: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G Expression is present in muscle precursors.
Specimen 3G': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G' Expression is present in muscle precursors.
Specimen 3J: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J Expression is present in muscle precursors.
Specimen 3J': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J' Expression is present in muscle precursors.
Specimen 3K: embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: ulnar nerve Present 3K
TS20: forelimb mesenchyme Absent 3K
Specimen 3K': embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: red - Pax3; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Absent 3K'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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