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Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5461573
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Reporter: lacZ
Antibody: anti-B-galactosidase (Cappel/ICN/MP Biomedicals)
Detection system: Secondary antibody coupled to Cy3
Assay notes: The authors described using three antibodies but did not specify which was used in the pictured assay; it is possible either MGI:3575669 or MGI:3529591 was used to generate some or all of these data.
Results
Specimen 3D: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3D Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
TS17: trunk dermomyotome Present Regionally restricted 3D Expression is detected in the ventrolateral lip.
TS17: trunk myotome Present 3D
TS17: body wall mesenchyme Present 3D Expression is present in the ventral somatopleure.
TS17: embryo mesenchyme Present Regionally restricted 3D Expression is present in the dorsal somatopleure.
Specimen 3E: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3E Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
TS17: trunk dermomyotome Present Regionally restricted 3E Expression is detected in the ventrolateral lip.
TS17: trunk myotome Present 3E
TS17: body wall mesenchyme Present 3E Expression is present in the ventral somatopleure.
TS17: embryo mesenchyme Present Regionally restricted 3E Expression is present in the dorsal somatopleure.
Specimen 3F: embryonic day 10.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS17: trunk myotome Present Widespread 3F Expression is present in the myotome and in migrating limb muscle precursors; some are shown at higher magnification in the inset.
Specimen 3G: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G Expression is present in muscle precursors.
Specimen 3G': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3G' Expression is present in muscle precursors.
Specimen 3H: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H Expression is present in muscle precursors.
Specimen 3H': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2; red - Myod1 (assay MGI:5461569).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H' Expression is present in muscle precursors.
Specimen 3I: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pitx2 protein (assay MGI:5461655).
Structure Level Pattern Image Note
TS20: forelimb Present 3I The endogenous protein and lacZ reporter protein co-localize.
Specimen 3I': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: green - Pitx2 reporter; red - Pitx2 protein (assay MGI:5461655).
Structure Level Pattern Image Note
TS20: forelimb Present 3I' The endogenous protein and lacZ reporter protein co-localize.
Specimen 3J: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J Expression is present in muscle precursors.
Specimen 3J': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3J' Expression is present in muscle precursors.
Specimen 3K: embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb Absent 3K
TS20: ulnar nerve Absent 3K
Specimen 3K': embryonic day 12.5; Pax3Sp-d/Pax3Sp-d, Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Double labeled: green - Pitx2; red - Pax3 (assay MGI:5461520).
Structure Level Pattern Image Note
TS20: forelimb Absent 3K'
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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