About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:117808 Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5461569
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Antibody: anti-MyoD
Detection system: Secondary antibody coupled to Cy5
Assay notes: The authors described using two antibodies but did not specify which was used in the pictured assay; it is possible MGI:2673272 was used to generate some or all of these data.
Results
Specimen 3E: embryonic day 10.25; Pitx2tm1Rsd/Pitx2+ (more )
Note: Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS17: limb mesenchyme Present 3E Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
Specimen 3H: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H Expression is present in muscle precursors.
Specimen 3H': embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present 3H' Expression is present in muscle precursors.
Specimen 4A: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: tongue muscle Present 4A
TS20: head mesenchyme Present Regionally restricted 4A Expression is present in the masseter anlagen.
Specimen 4C: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 4C Expression is present in the deep back muscle anlagen.
TS20: paraxial mesenchyme Present Regionally restricted 4C Expression is present in the intercostals muscle, latissimus dorsi and abdominal muscle anlagen.
TS20: limb mesenchyme Present Regionally restricted 4C Expression is present in the extensor and flexor muscle anlagen.
Specimen 4E: embryonic day 12.5; Pitx2tm1Rsd/Pitx2+ (more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Structure Level Pattern Image Note
TS20: hindlimb mesenchyme Present Regionally restricted 4E Expression is present in the muscle anlagen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory