Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:117808
Shih HP, et al., Expression pattern of the homeodomain transcription factor Pitx2 during muscle development. Gene Expr Patterns. 2007 Feb;7(4):441-51
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5461569
|
Gene symbol:
|
Myod1
|
Gene name:
|
myogenic differentiation 1
|
Antibody:
|
anti-MyoD
|
Detection system:
|
Secondary antibody coupled to Cy5
|
Assay notes:
|
The authors described using two antibodies but did not specify which was used in the pictured assay; it is possible MGI:2673272 was used to generate some or all of these data.
|
|
Results
|
Specimen
3E:
embryonic day 10.25; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Specimen
3E:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 10.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: limb mesenchyme |
Present |
|
3E
|
Expression is present in limb muscle precursors, as shown in higher magnification in the inset.
|
Specimen
3H:
embryonic day 12.5; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Specimen
3H:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
|
Specimen
3H':
embryonic day 12.5; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
Specimen
3H':
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Frontal section. Double labeled: red - Myod1; green - Pitx2 reporter (assay MGI:5461573).
|
Specimen
4A:
embryonic day 12.5; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
|
Specimen
4C:
embryonic day 12.5; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Specimen
4C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: trunk mesenchyme |
Present |
Regionally restricted |
4C
|
Expression is present in the deep back muscle anlagen.
|
TS20: paraxial mesenchyme |
Present |
Regionally restricted |
4C
|
Expression is present in the intercostals muscle, latissimus dorsi and abdominal muscle anlagen.
|
TS20: limb mesenchyme |
Present |
Regionally restricted |
4C
|
Expression is present in the extensor and flexor muscle anlagen.
|
Specimen
4E:
embryonic day 12.5; Pitx2tm1Rsd/Pitx2 +
(more )
Note: Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Pitx2tm1Rsd/Pitx2+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Transverse section. Triple labeled: red - Myod1; blue - Pitx2 reporter (assay MGI:5461570); green - Pax3 (assay MGI:5461523).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
4E
|
Expression is present in the muscle anlagen.
|
|