Probe:	MGF-10
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong	Regionally restricted	1B	High levels of expression in the dorsal thalamus.
TS25: telencephalon septum	Strong	Regionally restricted	1B	High levels of expression in the bed nucleus of stria terminalis.
TS25: corpus striatum	Present		1B	Lower levels of expression in the dentate gyrus and striatum.
TS25: dentate gyrus	Present		1B	Lower levels of expression in the dentate gyrus and striatum.
TS25: cortical plate	Present		1B
TS25: main olfactory bulb	Present		1B
TS25: hindbrain	Present	Regionally restricted	1B	Expression in the rhombencephalic lip.

Structure	Level	Pattern	Image	Note
TS24: brain	Present		1D	Authors report the pattern of expression in the brain was that same observed for normal embryos.
TS24: skin	Strong		1D
TS24: gut	Present		1D
TS24: lung	Present		1D
TS24: nasal cavity epithelium	Present		1D
TS24: testis	Present		1D

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Strong	Regionally restricted	2C left	Highest levels of expression in the the ventricular zone of the rostral telencephalon.
TS21: thalamus	Strong	Regionally restricted	2C left	High levels of expression in the anterior nuclei of the thalamus.
TS21: telencephalon	Strong	Regionally restricted	2C left	High levels of expression in the and bed nucleus of the stria terminalis.
TS21: corpus striatum	Trace		2C left	Faint expression in the corpus striatum.

Structure	Level	Pattern	Image	Note
TS21: embryo	Absent		2C right

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Strong		3B	High levels of expression in granulosa (follicular) cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		3E

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		3H left

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Strong		3H right	Markedly elevated levels of expression in Sertoli cells compared with wild type control.

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present		4B/F

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	4D/H maternal	Expression in the decidua in endothelial cells.

Structure	Level	Pattern	Image	Note
TS14: chorioallantoic placenta	Present	Regionally restricted	4D/H extraembryonic	Expression in the chorion and ectoplacental cone

Structure	Level	Pattern	Image	Note
TS21: labyrinthine zone	Present		4J

Structure	Level	Pattern	Image	Note
TS21: labyrinthine zone	Absent		4L

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present	Regionally restricted		Expression in neural-crest derived cranial ganglia.
TS20: dorsal root ganglion	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS23: cranial ganglion	Present	Regionally restricted		Expression in neural-crest derived cranial ganglia.
TS23: dorsal root ganglion	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		5B
TS24: skin	Present		5B

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		5D
TS24: skin	Present		5D

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	6B

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted	7B	Expression in two narrow bands of cells, one band just superficial to the mitral layer, the other band in the deeper side of the periglomerular region.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		7E
TS28: cortical layer III	Present		7E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Regionally restricted	8A

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Regionally restricted	8C	Authors state they were unable to observe any changes in expression relative to normal mice

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Regionally restricted	8E	Authors state they were unable to observe any changes in expression relative to normal mice

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		8G
TS28: entorhinal cortex	Strong		8G

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		8I	Authors state they were unable to observe any changes in expression relative to normal mice.
TS28: entorhinal cortex	Strong		8I	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		8K	Authors state they were unable to observe any changes in expression relative to normal mice.
TS28: entorhinal cortex	Strong		8K	Authors state they were unable to observe any changes in expression relative to normal mice.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted		Expression in internal tufted cells; probably external tufted cells.
TS28: cortical layer II	Strong
TS28: cortical layer III	Strong
TS28: corpus striatum	Present	Regionally restricted		Expression in neurons, probably in the matrix compartment.
TS28: telencephalon	Strong	Regionally restricted		Authors report expression in the paleocortex.
TS28: dentate gyrus	Strong	Regionally restricted		Expression in granular neurons of the dentate gyrus.
TS28: thalamus	Present	Regionally restricted		Very high expression in the lateral thalamus. Strong expression in the medial thalamus. Expression in the anterior thalamus.
TS28: hypothalamus	Absent
TS28: telencephalon septum	Present	Regionally restricted		Expression in the triangular nucleus.
TS28: brainstem	Present
TS28: red nucleus	Present
TS28: superior colliculus	Present
TS28: cerebellum Purkinje cell layer	Very strong	Regionally restricted		Expression in Purkinje neurons.
TS28: deep cerebellar nucleus	Present	Regionally restricted		Expression in lateral deep nuclei.