Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:103820 Reiter JF, et al., Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Genes Dev. 2006 Jan 1;20(1):22-7
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4461989
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:

anti-Pax6 (#PRB-278P; #30130)

Results

3B wild type ©

Specimen 3B wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B wild type

3B Tectonic-/- ©

Specimen 3B Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B Tectonic-/-	Expression was expanded compared to wild type.

3B Ptch-/- ©

Specimen 3B Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3B Ptch-/-

3B Tectonic-/- Ptch-/- ©

Specimen 3B Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Foxa2 (assay MGI:4462006).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3B Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3C wild type ©

Specimen 3C wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C wild type

3C Tectonic-/- ©

Specimen 3C Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C Tectonic-/-	Expression was expanded compared to wild type.

3C Ptch-/- ©

Specimen 3C Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3C Ptch-/-

3C Tectonic-/- Ptch-/- ©

Specimen 3C Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Nkx2-2 (assay MGI:4462009).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3C Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3D wild type ©

Specimen 3D wild type: embryonic day 9.5 (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D wild type

3D Tectonic-/- ©

Specimen 3D Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D Tectonic-/-	Expression was expanded compared to wild type.

3D Ptch-/- ©

Specimen 3D Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak		3D Ptch-/-

3D Tectonic-/- Ptch-/- ©

Specimen 3D Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Isl1 (assay MGI:4462015) and Isl2 (assay MGI:4462016).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D Tectonic-/- Ptch-/-	Expression was expanded compared to wild type.

3F wild type ©

Specimen 3F wild type: embryonic day 10.5 (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3F wild type	Expression was detected dorsally.

Specimen 3F Tectonic-/-: embryonic day 10.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3F Tectonic-/-	Expression was expanded compared to wild type.

Specimen 3F Rab23 opb/opb: embryonic day 10.5; Rab23^opb2/Rab23^opb2 (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3F Rab23 opb/opb	Expression was shifted dorsally compared to wild type.

Specimen 3F Tect-/- Rab23 opb/opb: embryonic day 10.5; Rab23^opb2/Rab23^opb2, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Pax3 (assay MGI:4462013).

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		3F Tect-/- Rab23 opb/opb	Expression was not shifted dorsally compared to wild type.

S4_wild type ©

Specimen S4 wild type: embryonic day 11.5 (more

)
Note: Double labeled: red - Pax6; green - Msx1 (assay MGI:4462020) and Msx2 (assay MGI:4462027).

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		S4_wild type

Specimen S4 Tectonic-/-: embryonic day 11.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: red - Pax6; green - Msx1 (assay MGI:4462020) and Msx2 (assay MGI:4462027).

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		S4_Tectonic-/-

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