Isl2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:103820 Reiter JF, et al., Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Genes Dev. 2006 Jan 1;20(1):22-7
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:4462016
Gene symbol:	Isl2
Gene name:	insulin related protein 2 (islet 2)

Antibody:	Anti-Islet1/2
Assay notes:	The antibody used in this assay recognizes both Isl1 and Isl2.

Results

3D wild type ©

Specimen 3D wild type: embryonic day 9.5 (more

)
Note: Double labeled: green - Isl1 and Isl2; red - Pax6 (assay MGI:4461989).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3D wild type	Expression was detected in motorneurons.

Specimen 3D Tectonic-/-: embryonic day 9.5; Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: green - Isl1 and Isl2; red - Pax6 (assay MGI:4461989).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak	Regionally restricted	3D Tectonic-/-	Expression was reduced in motorneurons.

3D Ptch-/- ©

Specimen 3D Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Double labeled: green - Isl1 and Isl2; red - Pax6 (assay MGI:4461989).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		3D Ptch-/-	Expression in the mutant was expanded compared to wild type.

Specimen 3D Tectonic-/- Ptch-/-: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps, Tctn1^{Gt(KST296)Byg}/Tctn1^{Gt(KST296)Byg} (more

)
Note: Double labeled: green - Isl1 and Isl2; red - Pax6 (assay MGI:4461989).

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak	Regionally restricted	3D Tectonic-/- Ptch-/-	Expression was reduced in motorneurons.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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