Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(H2-K-HLA-G,B2M)1Alm
Name:	transgene insertion 1, Andrew L Mellor
MGI ID:	MGI:3806366
Synonyms:	HLA-Gtg
Transgene:	Tg(H2-K-HLA-G,B2M)1Alm  Location: unknown
Alliance:	Tg(H2-K-HLA-G,B2M)1Alm page

Strain of Origin:

CBA/Ca

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence) Mutation:    Insertion   Tg(H2-K-HLA-G,B2M)1Alm expresses 2 genes Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human B2M (567) B2m  (MGI:88127) human HLA-G (3135) H2-D1  (MGI:95896) H2-K1  (MGI:95904) H2-M2  (MGI:95914) H2-M3  (MGI:95915) H2-M5  (MGI:95917) H2-M10.1  (MGI:1276522) H2-M10.2  (MGI:1276525) H2-M10.4  (MGI:1276527) H2-M10.6  (MGI:2687364) H2-M11  (MGI:2676637) H2-Q1  (MGI:95928) H2-Q2  (MGI:95931) H2-Q6  (MGI:95935) H2-Q7  (MGI:95936) H2-Q10  (MGI:95929) H2-T22  (MGI:95956)   Mutation details: Two transgenic constructs were co-injected into male pronuclei of CBA/Ca zygotes. The constructs encoded (i) a H-2Kb/HLA-G transgene with the human HLA-G heavy chain (HLA-G; major histocompatibility complex, class I, G) gene inserted between the mouse H-2Kb MHC class I promoter and coding sequence, and (ii) a human B2M transgene (designed with the human beta2-microglobulin gene). (A third transgene was also co-injected as well, but was found not to have integrated into the genome.) The mean copy number of HLA-G genomic DNA per haploid mouse genome was 3 copies. HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, thymus and kidney correlates better with Kb expression patterns. (J:140011, J:140016)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:140011 Warner CM, et al., Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online. 2008 Oct;17(4):470-85
All:	2 reference(s)