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Gene Expression Data
Assay Details
Assay
Reference: J:93502 Watanabe D, et al., Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages. Gene Expr Patterns. 2004 Nov;5(1):43-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3528300
Gene symbol: Dnmt3b
Gene name: DNA methyltransferase 3B
Antibody: anti-Dnmt3b
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen Not Shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: liver Present Single cells
Specimen 1E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: liver Present 1E
Specimen 1F: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: liver Present Single cells 1F
Specimen 1G: postnatal adult (more )
Structure Level Pattern Image Note
TS28: liver Absent 1G
Specimen 2A: embryonic day 11.5 (more )
Note: Double labeled: red - Dnmt3b; green - Cd34 (assay MGI:3528389).
Structure Level Pattern Image Note
TS19: liver Present Single cells 2A The cells expressing Dnmt3b expressed CD34, and vice versa.
Specimen 2C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: liver Present Single cells 2C
Specimen 2D: embryonic day 11.5 (more )
Note: Double labeled: green - Dnmt3b; red - Alb (assay MGI:3528426).
Structure Level Pattern Image Note
TS19: liver Present Single cells 2D Dnmt3b was not expressed in the cells that expressed albumin.
Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: red - Dnmt3b; green - Ly76 (assay MGI:3528427).
Structure Level Pattern Image Note
TS22: liver Present Single cells 2E
Specimen 2F: embryonic day 11.5 (more )
Note: Double labeled: green - Dnmt3b; red - Dnmt3a (assay MGI:3528428).
Structure Level Pattern Image Note
TS19: liver Present Single cells 2F The cells expressing Dnmt3b did not express a significant amount of Dnmt3a, and vice versa.
Specimen 3B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Single cells 3B
Specimen 3C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: dorsal aorta Present Single cells 3C
Specimen 3E,F: embryonic day 11.5 (more )
Note: Double labeled: red - Dnmt3b; green - Cd34 (assay MGI:3528389).
Structure Level Pattern Image Note
TS19: dorsal aorta Present Single cells 3F , 3E A few relatively large cells staying on the wall of E11.5 dorsal aorta were positively stained. These cells expressing Dnmt3b were also stained with CD34 antibodies.
Specimen 3H,I: embryonic day 11.5 (more )
Note: Double labeled: red - Dnmt3b; green - Cd34 (assay MGI:3528389).
Structure Level Pattern Image Note
TS19: blood Present Single cells 3H , 3I A few circulating cells in the portal vein were positively stained. These cells expressing Dnmt3b were also stained with CD34 antibodies.
TS19: portal vein Present Single cells 3H , 3I A few circulating cells in the portal vein were positively stained. These cells expressing Dnmt3b were also stained with CD34 antibodies.
Specimen 4A,C: embryonic day 8.0 (more )
Note: Double labeled: red - Dnmt3b; green - Itga2b (assay MGI:3528429).
Structure Level Pattern Image Note
TS12: embryo Present 4A , 4C
TS12: blood island Absent 4A , 4C
Specimen 4E: postnatal adult (more )
Note: Double labeled: red - Dnmt3b; green - Cd34 (assay MGI:3528389).
Structure Level Pattern Image Note
TS28: bone marrow Absent 4E
Specimen 5H,I: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5I , 5H Expression is in the nuclei of type A spermatogonia.
Specimen 5K: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5K Expression is in the nuclei of spermatogonia in both heterochromatin and euchromatin.
Specimen 5L: postnatal adult (more )
Note: Double labeled: green - Dnmt3b; red - Dnmt3a (assay MGI:3528428).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5L Dnmt3a and Dnmt3b were not significantly expressed in the same spermatogonia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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