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Gene Expression Data
Assay Details
Assay
Reference: J:93502 Watanabe D, et al., Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages. Gene Expr Patterns. 2004 Nov;5(1):43-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3528428
Gene symbol: Dnmt3a
Gene name: DNA methyltransferase 3A
Antibody: anti-Dnmt3a
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2F: embryonic day 11.5 (more )
Note: Double labeled: red - Dnmt3a; green - Dnmt3b (assay MGI:3528300).
Structure Level Pattern Image Note
TS19: liver Present 2F The cells expressing Dnmt3b did not express a significant amount of Dnmt3a, and vice versa.
Specimen 5E,F: postnatal adult (more )
Note: Double labeled: red - Dnmt3a; green - Dnmt3b (assay MGI:3528300).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 5F , 5E Expression is in the tubules at stages IV-VII.
TS28: spermatocyte Present Single cells 5E , 5F Expression in type-B spermatogonia and pre-leptotene spermatocytes.
TS28: spermatogonium Present Single cells 5E , 5F Expression in type-B spermatogonia and pre-leptotene spermatocytes.
Specimen 5J: postnatal adult (more )
Note: Double labeled: red - Dnmt3a; green - Dnmt3b (assay MGI:3528300).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5J Expression was localized in euchromatin of type-B spermatogonia cells.
Specimen 5L: postnatal adult (more )
Note: Double labeled: red - Dnmt3a; green - Dnmt3b (assay MGI:3528300).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5L Dnmt3a and Dnmt3b were not significantly expressed in the same spermatogonia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory