Antibody:	anti-TN1-123
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Present		2	Expression is located in the nucleus.
TS11: primitive streak	Present		2	Expression is located in the nucleus.
TS11: embryo mesoderm	Present		2	Expression is located in the nucleus of migrating mesoderm cells.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		3A

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		3B

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Present		3C
TS11: extraembryonic mesoderm	Present	Regionally restricted	3C	Expression is detectable in migrating extraembryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present		3D	Expression was detected in mesoderm.
TS13: embryo endoderm	Present		3D
TS13: neural ectoderm	Present		3D
TS13: notochord	Present		3D
TS13: notochordal plate	Present		3D

Structure	Level	Pattern	Image	Note
TS15: notochord	Present	Uniform	3E	Embryos show staining along the entire length of the notochord from the forebrain-midbrain boundary to the tailbud.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present	Uniform	3F	Embryos show staining along the entire length of the notochord from the forebrain-midbrain boundary to the tailbud.

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Weak	Regionally restricted	3G	Expression is weak in the anterior notochordal plate.
TS11: primitive streak	Weak		3G
TS11: node	Absent		3G	The node is absent in this mutant.

Structure	Level	Pattern	Image	Note
TS11: node	Strong

Structure	Level	Pattern	Image	Note
TS13: notochordal plate	Weak	Regionally restricted	3H	Expression is weak in the anterior notochordal plate.
TS13: primitive streak	Weak		3H
TS13: node	Absent		3H	The node is absent in this mutant.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		3I

Structure	Level	Pattern	Image	Note
TS11: node	Present		4A
TS11: notochordal plate	Strong		4A
TS11: primitive streak	Present	Regionally restricted	4A	Expression was detected in the primitive ectoderm layer of the primitive streak throughout its entire length.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	4B	Expression is in the migrating mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	4C	Along the primitive streak, expression appears higher in cells after ingression than in nascent mesodermal and endodermal cells in the primitive ectoderm. Laterally migrating cells, however, show decreasing levels of expression.

Structure	Level	Pattern	Image	Note
TS13: embryo ectoderm	Present	Non-Uniform	5A	All cells in the primitive embryonic ectoderm and in its vicinity, including prospective neuroectoderm cells, show expression, although at various levels.
TS13: neural ectoderm	Present	Non-Uniform	5A	All cells in the primitive embryonic ectoderm and in its vicinity, including prospective neuroectoderm cells, show expression, although at various levels.
TS13: gut endoderm	Present		5A

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present		5B
TS13: neural ectoderm	Present		5B
TS13: future spinal cord neural plate	Present	Non-Uniform	5B	Neural plate cells show various levels of residual expression.

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present		5C
TS13: neural ectoderm	Present		5C
TS13: notochordal plate	Present		5C	As differentiation proceeds, expression levels are increasing in the notochordal plate.
TS13: future spinal cord neural plate	Present	Non-Uniform	5C	Neural plate cells show various levels of residual expression.

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present		5D
TS13: notochordal plate	Present		5D	As differentiation proceeds, expression levels are increasing in the notochordal plate.

Structure	Level	Pattern	Image	Note
TS13: notochordal plate	Present		5E	As differentiation proceeds, expression levels are increasing in the notochordal plate.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		5F

Structure	Level	Pattern	Image	Note
TS13: notochordal plate	Weak		5G	Expression cells of the notochordal plate take an abnormal position on the ventral side of the neural plate and have a reduced level of expression compared to wild type cells at this stage.

Structure	Level	Pattern	Image	Note
TS13: notochordal plate	Weak		5H	Expression cells of the notochordal plate take an abnormal position on the ventral side of the neural plate and have a reduced level of expression compared to wild type cells at this stage.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	5I	The posterior primitive streak appears as a bulk of densely packed cells with some residual T protein.

Structure	Level	Pattern	Image	Note
TS19: tail	Strong		6A , 6B
TS19: notochord	Strong	Uniform	6B , 6A	Expression was detected in the entire notochord.

Structure	Level	Pattern	Image	Note
TS20: tail	Present		6D , 6C
TS20: notochord	Present	Graded	6D , 6C	There is a gradual decrease of staining from posterior to anterior in the tail notochord. Expression is absent from the notochord anterior to the base of the tail.

Structure	Level	Pattern	Image	Note
TS21: tail	Present

Structure	Level	Pattern	Image	Note
TS22: tail	Present

Structure	Level	Pattern	Image	Note
TS17: notochord	Present	Non-Uniform	6E	Staining in the notochord of the posterior trunk, the sacral region, is discontinuous.

Structure	Level	Pattern	Image	Note
TS19: tail	Present	Regionally restricted	6F	Expression in this mutant is the same as in wild-type embryos; however the tailbud frequently produces multiple apices.

Structure	Level	Pattern	Image	Note
TS20: tail	Weak	Regionally restricted	6G	Tailbud expression has almost vanished.
TS20: notochord	Present	Non-Uniform	6G	Only islands of notochord tissue are stained in the posterior extreme of the trunk of this particular embryo.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present	Non-Uniform	6I	Staining in the notochord of the posterior trunk, the sacral region, is discontinuous.

Structure	Level	Pattern	Image	Note
TS19: tail	Present	Regionally restricted	6J	Expression in this mutant is the same as in wild-type embryos; however the tailbud frequently produces multiple apices.

Structure	Level	Pattern	Image	Note
TS20: tail	Weak	Regionally restricted	6K	Tailbud expression has almost vanished.
TS20: notochord	Present	Non-Uniform	6K	Only islands of notochord tissue are stained in the posterior extreme of the trunk of this particular embryo.

Structure	Level	Pattern	Image	Note
TS17: tail	Present		7A

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	7B	Almost all cells of the tailbud, except for a surface layer, show expression.

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	7C	Almost all cells of the tailbud, except for a surface layer, show expression.
TS17: tail neural tube	Strong	Regionally restricted	7C	The dorsal aspect of the neural tube is strongly stained.

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	7D	Almost all cells of the tailbud, except for a surface layer, show expression.
TS17: notochord	Present		7D
TS17: embryo mesenchyme	Weak		7D	The neural tube, gut and surroudning mesodermal tissue show weaker staining than the differentiating notochord.
TS17: gut	Weak		7D	The neural tube, gut and surroudning mesodermal tissue show weaker staining than the differentiating notochord.
TS17: tail neural tube	Weak		7D	The neural tube, gut and surroudning mesodermal tissue show weaker staining than the differentiating notochord.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		7E

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present		7F	Gut, neural tube, and surrounding cells are stained.
TS17: gut	Present		7F	Gut, neural tube, and surrounding cells are stained.
TS17: tail neural tube	Present		7F	Gut, neural tube, and surrounding cells are stained.
TS17: notochord	Absent		7F	The notochord is not detectable in the mutant.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present	Regionally restricted	7G	In more anterior regions to those in Fig. 7F, expressing notochord-like cells were found integrated in a malformed neural tube.