Pkm MGI Mouse Gene Detail - MGI:97591 - pyruvate kinase, muscle

Symbol

Pkm
Name

pyruvate kinase, muscle
Synonyms

Pk-2, Pk-3, Pk3, Pkm2

Feature Type

protein coding gene
IDs

MGI:97591
NCBI Gene: 18746
Alliance

gene page
Transcription Start Sites

16 TSS
Regulated by

Rr565 (1 regulatory region)

Sequence Map

Chr9:59563859-59586655 bp, + strand
From NCBI annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 9, 32.03 cM
Mapping Data

14 experiments

SNPs within 2kb

1197 from dbSNP Build 142
Strain Annotations

16

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_97591	protein coding gene	Chr9:59563651-59586658 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0034904	protein coding gene	Chr9:59017056-59041467 (+)
A/J	no annotation
AKR/J	MGP_AKRJ_G0034814	protein coding gene	Chr9:58440721-58469720 (+)
BALB/cJ	MGP_BALBcJ_G0034877	protein coding gene	Chr9:56857371-56880378 (+)
C3H/HeJ	MGP_C3HHeJ_G0034587	protein coding gene	Chr9:58936228-58959091 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0035396	protein coding gene	Chr9:60649361-60672356 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0032307	protein coding gene	Chr9:56857085-56880199 (+)
CAST/EiJ	MGP_CASTEiJ_G0033908	protein coding gene	Chr9:59026509-59049439 (+)
CBA/J	MGP_CBAJ_G0034558	protein coding gene	Chr9:62791832-62817638 (+)
DBA/2J	MGP_DBA2J_G0034717	protein coding gene	Chr9:56940990-56963722 (+)
FVB/NJ	MGP_FVBNJ_G0034659	protein coding gene	Chr9:56201616-56224780 (+)
LP/J	MGP_LPJ_G0034800	protein coding gene	Chr9:59320722-59343561 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0034702	protein coding gene	Chr9:64225307-64249263 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0035418	protein coding gene	Chr9:58452887-58477464 (+)
PWK/PhJ	MGP_PWKPhJ_G0033612	protein coding gene	Chr9:57002107-57025915 (+)
SPRET/EiJ	no annotation
WSB/EiJ	MGP_WSBEiJ_G0034021	protein coding gene	Chr9:58398341-58426950 (+)

Human Ortholog

PKM, pyruvate kinase M1/2

Vertebrate Orthologs

4

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

PKM, pyruvate kinase M1/2
Synonyms

CTHBP, HEL-S-30, OIP3, p58, PK3, PKM2, TCB, THBP1
Links

HGNC:9021

NCBI Gene ID: 5315

UniProt: P14618
Chr Location

15q23; chr15:72199029-72231819 (-) GRCh38

MGI Vertebrate Homology

Pkm stringent orthology
1 human;1 mouse;1 rat;2 zebrafish
HCOP

vertebrate homology predictions: PKM
Gene Tree

Pkm

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Phenotype Summary

13 phenotypes from 3 alleles in 4 genetic backgrounds
4 phenotypes from multigenic genotypes
1 images
76 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

150
Endonuclease-mediated

14
Gene trapped

128
Spontaneous

1
Targeted

5
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

80 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Mice homozygous for a knock-out allele exhibit decreased embryo size, absent egg cylinder, failure of primitive streak formation, absent primitive node and head folds, failure to gastrulate, and complete embryonic lethality by E9.5. Mice homozygous for a spontaneous allele exhibit prenatal lethality around the time of implantation.

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All GO Annotations

32
GO References

13

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

953
Tissues

123
cDNA Data

33
Literature Summary

32

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

GEISHA PKLR

Xenbase pkm

ZFIN pkma, pkmb

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All Sequences

168
RefSeq

32
UniProt

8
CCDS

CCDS40659.1, CCDS57681.1

Ensembl

ENSMUSG00000032294 (Evidence)
NCBI Gene

18746

			Length	Strain/Species	Flank
genomic	18746	NCBI Gene Model \| MGI Sequence Detail	22797	C57BL/6J	± kb
transcript	NM_001378871	RefSeq \| MGI Sequence Detail	3587	ZRU/MplStud
polypeptide	P52480	UniProt \| EBI \| MGI Sequence Detail	531	Not Applicable

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UniProt

8 Sequences
Protein Ontology

PR:000012783 pyruvate kinase PKM

(term hierarchy)
EC

2.7.1.40, 2.7.10.2, 2.7.11.1
InterPro Domains

IPR001697 Pyruvate kinase

IPR018209 Pyruvate kinase, active site

IPR015793 Pyruvate kinase, barrel

IPR015795 Pyruvate kinase, C-terminal

IPR036918 Pyruvate kinase, C-terminal domain superfamily

IPR015806 Pyruvate kinase, insert domain superfamily

IPR040442 Pyruvate kinase-like domain superfamily

IPR011037 Pyruvate kinase-like, insert domain superfamily

IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily
GlyGen

P52480 2 sites, 1 O-linked glycan (2 sites)

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All nucleic 44

cDNA 34

Primer pair 10

Microarray probesets 3

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MGD-MRK-13319, MGD-MRK-13320, MGD-MRK-13322, MGI:2143183, MGI:2143184, MGI:2144733

Summaries

All 189

Developmental Gene Expression 32

Gene Ontology 13

Phenotypes 76
Earliest

J:5994 Lalley PA, et al., Conservation of autosomal gene synteny groups in mouse and man. Nature. 1978 Jul 13;274(5667):160-3
Latest

J:361525 Wu M, et al., PKM2 controls cochlear development through lactate-dependent transcriptional regulation. Proc Natl Acad Sci U S A. 2025 Jan 14;122(2):e2410829122

Pkm expression regulated by:

Feature	Feature Type	Location	Reference
Rr565, regulatory region 565	response element	Chr9, Syntenic	J:358467

TSS for Pkm:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr85450	Chr9:59563455-59563458 (+)	-402 bp
Tssr85451	Chr9:59563831-59563867 (+)	-10 bp
Tssr85452	Chr9:59563874-59563882 (+)	19 bp
Tssr85453	Chr9:59564046-59564138 (+)	233 bp
Tssr85454	Chr9:59564387-59564405 (+)	537 bp
Tssr85455	Chr9:59564483-59564535 (+)	650 bp
Tssr85456	Chr9:59564807-59564864 (+)	977 bp
Tssr85457	Chr9:59564898-59564914 (+)	1,047 bp
Tssr85458	Chr9:59565743-59565772 (+)	1,899 bp
Tssr85459	Chr9:59566886-59566892 (+)	3,030 bp
Tssr85460	Chr9:59568743-59568795 (+)	4,910 bp
Tssr85461	Chr9:59572440-59572477 (+)	8,600 bp
Tssr85462	Chr9:59572588-59572612 (+)	8,741 bp
Tssr85463	Chr9:59576187-59576204 (+)	12,337 bp
Tssr85464	Chr9:59584189-59584212 (+)	20,342 bp
Tssr85465	Chr9:59584354-59584369 (+)	20,503 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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