Spink8 Gene Detail

Summary

• Symbol
  Spink8
• Name
  serine peptidase inhibitor, Kazal type 8
• Synonyms
  C630041L24Rik

• Feature Type
  protein coding gene
• IDs
  MGI:1925959
  NCBI Gene: 78709
• Alliance
  gene page
• Transcription Start Sites
  10 TSS

Location &
Maps

• Sequence Map
  Chr9:109641348-109655701 bp, + strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 9, 59.77 cM
• Mapping Data
  1 experiment

Strain
Comparison

• SNPs within 2kb
  615 from dbSNP Build 142
• Strain Annotations
  18

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1925959	protein coding gene	Chr9:109641271-109655701 (+)
129S1/SvImJ	MGP_129S1SvImJ_G0035336	protein coding gene	Chr9:111903884-111919729 (+)
A/J	MGP_AJ_G0035317	protein coding gene	Chr9:107860347-107874362 (+)
AKR/J	MGP_AKRJ_G0035248	protein coding gene	Chr9:110533323-110547210 (+)
BALB/cJ	MGP_BALBcJ_G0035312	protein coding gene	Chr9:107596430-107609388 (+)
C3H/HeJ	MGP_C3HHeJ_G0035020	protein coding gene	Chr9:110997315-111010626 (+)
C57BL/6NJ	MGP_C57BL6NJ_G0035834	protein coding gene	Chr9:114949890-114965166 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0032720	protein coding gene	Chr9:105100324-105113388 (+)
CAST/EiJ	MGP_CASTEiJ_G0034326	protein coding gene	Chr9:110806624-110823400 (+)
CBA/J	MGP_CBAJ_G0034987	protein coding gene	Chr9:119216087-119230040 (+)
DBA/2J	MGP_DBA2J_G0035146	protein coding gene	Chr9:107320988-107334378 (+)
FVB/NJ	MGP_FVBNJ_G0035093	protein coding gene	Chr9:106150238-106164483 (+)
LP/J	MGP_LPJ_G0035225	protein coding gene	Chr9:112218900-112232185 (+)
NOD/ShiLtJ	MGP_NODShiLtJ_G0035133	protein coding gene	Chr9:120592386-120605646 (+)
NZO/HlLtJ	MGP_NZOHlLtJ_G0035854	protein coding gene	Chr9:110830814-110843815 (+)
PWK/PhJ	MGP_PWKPhJ_G0034033	protein coding gene	Chr9:107543425-107556723 (+)
SPRET/EiJ	MGP_SPRETEiJ_G0033868	protein coding gene	Chr9:110915059-110928461 (+)
WSB/EiJ	MGP_WSBEiJ_G0034447	protein coding gene	Chr9:110721169-110734878 (+)

Homology

• Human Ortholog
  SPINK8, serine peptidase inhibitor Kazal type 8 (putative)

• Vertebrate Orthologs
  2

Vertebrate Orthology Source

Alliance of Genome Resources

• Human Ortholog
  SPINK8, serine peptidase inhibitor Kazal type 8 (putative)
  
• Links
  HGNC:33160

  NCBI Gene ID: 646424

  UniProt: P0C7L1

  
  
• Chr Location
  3p21.31; chr3:48306842-48333661 (-)  GRCh38

---

• MGI Vertebrate Homology
  Spink8 stringent orthology
  1 human;1 mouse;1 rat
  
• HCOP
  vertebrate homology predictions: SPINK8
• Gene Tree
  Spink8
  

Mutations,
Alleles, and
Phenotypes

• Phenotype Summary
  1 phenotype from 2 alleles in 1 genetic background
  5 phenotype references
  

• All Mutations and Alleles
  5
• Endonuclease-mediated
  2
• Targeted
  3
• Incidental Mutations
  APF
• Find Mice (IMSR)
  10 strains or lines available

Male mice homozygous for a mutation are viable and show normal fertility.

Expression

• Assay Results
  735
• Tissues
  27
• cDNA Data
  11
• Literature Summary
  1

Tissue x Stage Matrix

• Other Mouse Links
  Allen Institute
  GEO
  Expression Atlas

Sequences &
Gene Models

• All Sequences
  40
• RefSeq
  4
• UniProt
  3
• CCDS
  CCDS40777.1

• Ensembl
  ENSMUSG00000050074 (Evidence)
• NCBI Gene
  78709

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000050074	Ensembl Gene Model | MGI Sequence Detail	14354	C57BL/6J
transcript	ENSMUST00000198988	Ensembl | MGI Sequence Detail	768	Not Applicable
polypeptide	ENSMUSP00000142586	Ensembl | MGI Sequence Detail	105	Not Applicable

Protein
Information

• UniProt
  3 Sequences
• Protein Ontology
  PR:000015539 serine protease inhibitor Kazal-type 8
  (term hierarchy)
• InterPro Domains
  IPR002350 Kazal domain
  IPR036058 Kazal domain superfamily

Molecular
Reagents

• All nucleic 12

  cDNA 11

  Primer pair 1

  
  

  Microarray probesets 3

References

• Summaries
  All 23

  Developmental Gene Expression 1

  Phenotypes 5
• Earliest
  J:65060 Kawai J, et al., Functional annotation of a full-length mouse cDNA collection. Nature. 2001 Feb 8;409(6821):685-90
• Latest
  J:234235 Miyata H, et al., Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10

TSS for Spink8:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr87237	Chr9:109641275-109641286 (+)	-67 bp
Tssr87238	Chr9:109641287-109641298 (+)	-55 bp
Tssr87239	Chr9:109641317-109641337 (+)	-21 bp
Tssr87240	Chr9:109641357-109641368 (+)	15 bp
Tssr87241	Chr9:109645606-109645617 (+)	4,264 bp
Tssr87242	Chr9:109645647-109645655 (+)	4,303 bp
Tssr87243	Chr9:109645656-109645683 (+)	4,322 bp
Tssr87244	Chr9:109645686-109645707 (+)	4,349 bp
Tssr87245	Chr9:109645711-109645737 (+)	4,376 bp
Tssr87246	Chr9:109645743-109645767 (+)	4,407 bp