Gal3st1 MGI Mouse Gene Detail - MGI:1858277 - galactose-3-O-sulfotransferase 1

Symbol

Gal3st1
Name

galactose-3-O-sulfotransferase 1
Synonyms

3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase, Cst, galactosylceramide sulfotransferase, GalCer sulfotransferase, Gcst

Feature Type

protein coding gene
IDs

MGI:1858277
NCBI Gene: 53897
Alliance

genome feature page
Transcription Start Sites

5 TSS

Sequence Map

Chr11:3933636-3949326 bp, + strand
From Ensembl annotation of GRCm39
View this region in JBrowse
Genome Browsers

Ensembl | UCSC | NCBI

Genetic Map

Chromosome 11, 2.81 cM, cytoband A1
Mapping Data

3 experiments

SNPs within 2kb

395 from dbSNP Build 142
Strain Annotations

19

For selected strains:

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1858277	protein coding gene	Chr11:3933636-3949328 (+)
129S1/SvImJ	ENSMUSG00200029756	protein coding gene	Chr11:887760-903426 (+)
A/J	ENSMUSG00195021361	protein coding gene	Chr11:795016-810692 (+)
AKR/J	ENSMUSG00220008000	protein coding gene	Chr11:808084-823766 (+)
BALB/cJ	ENSMUSG00180022144	protein coding gene	Chr11:817798-833475 (+)
C3H/HeJ	ENSMUSG00175009245	protein coding gene	Chr11:807589-823279 (+)
C57BL/6NJ	ENSMUSG00215022373	protein coding gene	Chr11:817977-833673 (+)
CAROLI/EiJ	MGP_CAROLIEiJ_G0016022	protein coding gene	Chr11:802812-819663 (+)
CAST/EiJ	ENSTCUG00005036624	protein coding gene	Chr11:907414-922837 (+)
CBA/J	ENSMUSG00210017881	protein coding gene	Chr11:807189-822878 (+)
DBA/2J	ENSMUSG00185017543	protein coding gene	Chr11:904938-920628 (+)
FVB/NJ	ENSMUSG00205033548	protein coding gene	Chr11:783580-799254 (+)
JF1/MsJ	ENSUMUG00000036884	protein coding gene	Chr11:1067450-1082859 (+)
LP/J	ENSMUSG00230033844	protein coding gene	Chr11:1970919-1986585 (+)
NOD/ShiLtJ	ENSMUSG00190030628	protein coding gene	Chr11:794774-810450 (+)
NZO/HlLtJ	ENSMUSG00225025707	protein coding gene	Chr11:4027688-4043370 (+)
PWK/PhJ	ENSLUMG00010018731	protein coding gene	Chr11:795090-810758 (+)
SPRET/EiJ	ENSMSPG00010008497	protein coding gene	Chr11:888198-903601 (+)
WSB/EiJ	ENSIUOG00005006503	protein coding gene	Chr11:808488-824159 (+)

Human Ortholog

GAL3ST1, galactose-3-O-sulfotransferase 1

Vertebrate Orthologs

4

Vertebrate Orthology Source

Alliance of Genome Resources

Human Ortholog

GAL3ST1, galactose-3-O-sulfotransferase 1
Synonyms

CST
Links

HGNC:24240

NCBI Gene ID: 9514

UniProt: Q99999
Chr Location

22q12.2; chr22:30554632-30576210 (-) GRCh38

MGI Vertebrate Homology

Gal3st1 stringent orthology
1 human;1 mouse;1 rat;2 zebrafish
HCOP

vertebrate homology predictions: GAL3ST1
Gene Tree

Gal3st1

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Phenotype Summary

43 phenotypes from 3 alleles in 3 genetic backgrounds
9 phenotypes from multigenic genotypes
38 phenotype references

Phenotype Overview

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene.

adipose tissue	behavior/neurological	cardiovascular system	cellular	craniofacial	digestive/alimentary system	embryo	endocrine/exocrine glands	growth/size/body	hearing/vestibular/ear	hematopoietic system	homeostasis/metabolism	integument	immune system	limbs/digits/tail	liver/biliary system	mortality/aging	muscle	nervous system	pigmentation	renal/urinary system	reproductive system	respiratory system	skeleton	taste/olfaction	neoplasm	vision/eye

Click cells to view annotations.

All Mutations and Alleles

10
Endonuclease-mediated

4
Gene trapped

1
Targeted

4
Transgenic

1
Incidental Mutations

Mutagenetix , APF , CvDC
Find Mice (IMSR)

20 strains or lines available
Comparison Matrix

Gene Expression + Phenotype

Homozygotes for a targeted null mutation exhibit hindlimb weakness and progressive ataxia beginning at six weeks of age. Homozygous males exhibit sterility with a block in spermatogenesis prior to the first meiotic division.

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All GO Annotations

28
GO References

13

Molecular Function

carbohydrate derivative binding	cytoskeletal protein binding	DNA binding	enzyme regulator	hydrolase	ligase	lipid binding	oxidoreductase	RNA binding	signaling receptor activity	signaling receptor binding	transcription	transferase	transporter

Biological Process

carbohydrate derivative metabolism	cell differentiation	cell population proliferation	cellular component organization	DNA-templated transcription	establishment of localization	homeostatic process	immune system process	lipid metabolic process	programmed cell death	protein metabolic process	response to stimulus	signaling	system development

Cellular Component

cell projection	cytoplasmic vesicle	cytoskeleton	cytosol	endoplasmic reticulum	endosome	extracellular region	Golgi apparatus	mitochondrion	membraneless organelle	nucleus	organelle envelope	organelle lumen	plasma membrane	protein-containing complex	synapse	vacuole

Click cells to view annotations.

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Expression Overview

GXD's primary emphasis is on endogenous gene expression during development. Click on grid cells to view annotations.

Blue cells = expressed in wild-type.
Gray triangles = other expression annotations only
(e.g. absence of expression or data from mutants).

early conceptus	embryo ectoderm	embryo endoderm	embryo mesoderm	embryo mesenchyme	extraembryonic component	alimentary system	auditory system	branchial arches	cardiovascular system	connective tissue	endocrine system	exocrine system	hemolymphoid system	integumental system	limbs	liver and biliary system	musculoskeletal system	nervous system	olfactory system	reproductive system	respiratory system	urinary system	visual system

Click cells to view annotations.

Assay Results

726
Tissues

111
cDNA Data

9
Literature Summary

4

Images

Tissue x Stage Matrix

Comparison Matrix

Gene Expression + Phenotype

Other Mouse Links

Allen Institute

GEO

Expression Atlas
Other Vertebrate Links

Xenbase gal3st1

ZFIN gal3st1a, gal3st1b

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All Sequences

52
RefSeq

16
UniProt

1
CCDS

CCDS24373.1

Ensembl

ENSMUSG00000049721 (Evidence)
NCBI Gene

53897

			Length	Strain/Species	Flank
genomic	ENSMUSG00000049721	Ensembl Gene Model \| MGI Sequence Detail	15691	C57BL/6J	± kb
transcript	ENSMUST00000078757	Ensembl \| MGI Sequence Detail	1749	Not Applicable
polypeptide	ENSMUSP00000077815	Ensembl \| MGI Sequence Detail	423	Not Applicable

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UniProt

1 Sequence
Protein Ontology

PR:000007807 galactosylceramide sulfotransferase

(term hierarchy)
EC

2.8.2.11
InterPro Domains

IPR009729 Galactose-3-O-sulfotransferase

IPR027417 P-loop containing nucleoside triphosphate hydrolase
GlyGen

Q9JHE4 3 sites

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All nucleic 11

cDNA 9

Primer pair 2

Microarray probesets 2

Summaries

All 72

Developmental Gene Expression 4

Gene Ontology 13

Phenotypes 38
Earliest

J:289085 Honke K, et al., Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase. J Biol Chem. 1997 Feb 21;272(8):4864-8
Latest

J:384155 He S, et al., Sulfatide deficiency-induced astrogliosis and myelin lipid dyshomeostasis are independent of TREM2-mediated microglial activation. Nat Commun. 2026 Jan 9;17(1):338

TSS for Gal3st1:

(View these features in JBrowse)

Transcription Start Site	Location	Distance from Gene 5'-end
Tssr100076	Chr11:3933640-3933695 (+)	32 bp
Tssr100077	Chr11:3933704-3933737 (+)	85 bp
Tssr100078	Chr11:3933740-3933758 (+)	113 bp
Tssr100079	Chr11:3939924-3939946 (+)	6,299 bp
Tssr100080	Chr11:3942489-3942524 (+)	8,871 bp

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 09/08/2026 MGI 6.24