Zfp125 Gene Detail

Summary

• Symbol
  Zfp125
• Name
  zinc finger protein 125
• Synonyms
  ZT2

• Feature Type
  protein coding gene
  BioType Conflict
  
• IDs
  MGI:1336211
  NCBI Gene: 22651
• Alliance
  gene page
• Candidate for QTL
  1 QTL

Location &
Maps

• Sequence Map
  Chr12:20948575-20952660 bp, - strand

  From Ensembl annotation of GRCm39
  

• View this region in JBrowse
  

• Genome Browsers
  Ensembl | UCSC | NCBI

• Genetic Map
  Chromosome 12, 8.28 cM
• Mapping Data
  3 experiments

Strain
Comparison

• SNPs within 2kb
  77 from dbSNP Build 142
• Strain Annotations
  1
• PCR
  1

Strain	Gene Model ID	Feature Type	Coordinates
C57BL/6J	MGI_C57BL6J_1336211	protein coding gene	Chr12:20948575-20952660 (-)
129S1/SvImJ	no annotation
A/J	no annotation
AKR/J	no annotation
BALB/cJ	no annotation
C3H/HeJ	no annotation
C57BL/6NJ	no annotation
CAROLI/EiJ	no annotation
CAST/EiJ	no annotation
CBA/J	no annotation
DBA/2J	no annotation
FVB/NJ	no annotation
LP/J	no annotation
NOD/ShiLtJ	no annotation
NZO/HlLtJ	no annotation
PWK/PhJ	no annotation
SPRET/EiJ	no annotation
WSB/EiJ	no annotation

Homology

• Gene Tree
  Zfp125
  

Mutations,
Alleles, and
Phenotypes

• Incidental Mutations
  Mutagenetix , APF
• Find Mice (IMSR)
  2 strains or lines available

Gene Ontology
(GO)
Classifications

• All GO Annotations
  9
• GO References
  1

Expression

• Assay Results
  733
• Tissues
  12
• cDNA Data
  3
• Literature Summary
  1

Tissue x Stage Matrix

• Other Mouse Links
  GEO
  Expression Atlas

Sequences &
Gene Models

• All Sequences
  5
• UniProt
  1

• Ensembl
  ENSMUSG00000069755 (Evidence)
• NCBI Gene
  22651

Representative Sequences			Length	Strain/Species
genomic	ENSMUSG00000069755	Ensembl Gene Model | MGI Sequence Detail	4086	C57BL/6J
transcript	ENSMUST00000079237	Ensembl | MGI Sequence Detail	2488	Not Applicable
polypeptide	O89005	UniProt | EBI | MGI Sequence Detail	179	Not Applicable

Protein
Information

• UniProt
  1 Sequence
• InterPro Domains
  IPR036236 Zinc finger C2H2 superfamily
  IPR013087 Zinc finger C2H2-type

Molecular
Reagents

• All nucleic 5

  cDNA 3

  Primer pair 2

  
  

  Microarray probesets 3

References

• Summaries
  All 13

  Developmental Gene Expression 1

  Gene Ontology 1
• Earliest
  J:54688 Giorgi S, et al., Isolation and characterization of the murine zinc finger coding gene, ZT2: expression in normal and transformed myogenic cells. Gene. 1999 Apr 1;230(1):81-90
• Latest
  J:270723 Fernandes GW, et al., The Foxo1-Inducible Transcriptional Repressor Zfp125 Causes Hepatic Steatosis and Hypercholesterolemia. Cell Rep. 2018 Jan 9;22(2):523-534

Zfp125 is Candidate Gene for:

QTL	Genetic Location*	Genome Location (GRCm39)	Reference	QTL Note
Fl1sa	Chr12, 13.69 cM		J:244215	Nonalcoholic fatty liver disease (NAFLD) is a multifactorial disease caused by interactions between environmental and genetic factors. It was previously determined that SMXA-5 mice develop severe fatty liver on a high fat diet, although parental SM/J and A/J mice were resistant to fatty liver. Quantitative trait analysis using (SM/J x SMxA-5)F2 intercrossed mice identified a significant QTL, Fl1sa (fatty liver 1 in SMXA), for liver weight, liver triglyceride and total lipid content on centromere-53.06 Mb of mouse Chromosome 12. The A/J allele at Fl1sa was associated with the fatty liver. [J:124974] In a subsequent study using the livers from A/J-Chr 12 consomic and A/J mice that were fed a high fat diet three candidate genes were identified, Iah1, Rrm2 and Prkd1 using DNA microarray analysis. [J: 240255] The goal of the current study was to narrow the previously identified Fl1sa region by genetic dissection using novel congenic mice to identify candidate genes within the narrowed Fl1sa region. Two strains of congenic mice, A.SM-(centromere - D12Mit85)/J (R2) and A.SM-(D12Mit85-D12Mit112)/J (R3), were constructed from parental A/J and A/JChr 12 . Microsatellite markers and SNPs were used for the genotyping of R2 and R3 genomic DNA. Physical positions were taken from GRCm38.P4. The lipid accumulation in the respective livers was analyzed. As the liver triglyceride content in R2 and R3 congenic mice was significantly lower than in A/J mice. Male A/J, A/J-Chr12, R2 and R3 mice were fed standard chow until 6 weeks of age and thereafter fed a high fat diet from 6 to 13 weeks of age. At 13 weeks of age all mice were sacrificed after a 4 hour fast. The liver and white adipose tissue (subcutaneous fat, epididymal fat, mesenteric fat and retroperitoneal fat) were harvested and weighed. Blood sample were collected to measure serum lipids. Serum triglyceride, cholesterol and hepatic triglyceride content were measured. Whole transcripts from epididymal fats were measured using a Mouse Genome ST 2.0 array. Liver triglyceride content was significantly lower in A/J-Chr 12, R2 and R3 mice compared with that of A/J mice. Liver triglyceride and liver total lipid in R2 and R3 mice showed intermediate values between those of A/J and A/J-Chr 12 mice. The results suggested that the genes responsible for fatty liver existed in the SM/J region in strain R2 (centromere-29.20 Mb) and in the SM/J region in strain R3 (29.20-46.75 Mb) of Chromosome 12, Fig 1. Subsequently, in both of the narrowed Fl1sa regions microarray analysis of liver and epididymal fat from A/J and A/J-Chr 12 mice was performed in an attempt to identify candidate genes. Significant differences in gene expression levels in epididymal fat analysis were confirmed in 6 candidate genes: Ntsr2, Zfp125, Gdap10, Nrcam, Stxbp6 and Nova1 between the two strains.

*cM position of peak correlated region/marker