Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  7
• Reference
  J:36502 Butler A, et al., Mapping of the mouse serum amyloid A gene cluster by long-range polymerase chain reaction. Immunogenetics. 1996;44(6):468-74
• ID
  MGI:47853

Genes

Gene	Assay Type	Description
Saa1	overlapping clones	MSAA1LR/MSAA2LR PCR, MSAA1LF/MSAA4LR PCR
Saa2	overlapping clones	MSAA1LR/MSAA2LR PCR
Saa3	overlapping clones	MSAA5LF/MSAA3LR PCR
Saa-ps	overlapping clones	MSAA1LF/MSAA4LR PCR, MSAA4LF/MSAA5LR'B PCR
Saa4	overlapping clones	MSAA4LF/MSAA5LR'B PCR, MSAA5EcoRIF/MSAA5EcoRIR PCR, MSAA5LF/MSAA3LR PCR

Notes

• Reference
  Authors use Saa4 and Saa5 for Saa-ps and Saa4 respectively.
  
• Experiment
  Combinations of interlocus PCR primers were used to generate overlapping long-range PCR products to produce a complete map of the Saa genes on mouse Chromosome 7. The order of genes in the linkage group was reported to be 3' - Saa2 - 5'/5' - Saa1 - 3'/5' - Saa-ps - 3'/5' - Saa4 - 3'/5' - Saa3 - 3'. Authors indicate an Saa1 - Saa-ps intragenic distance of approximately 5.1kb. A distance of 9.3 - 9.4kb was reported to separate transcriptional start sites of the mouse Saa1 and Saa2 genes. The five mouse genes are within 45kb of each other.