Experiment
Genetic loci associated with long bone length, necropsy body weight, and organ weight were mapped in 1045 animals from a (LG/J x SM/J)F2 intercross. Animals were phenotyped between 70 and 237 days of age, with an average age at sacrifice of 144 days. Twodifferent sets of F2 mice were genotyped for 75 and 96 polymorphic markers across 19 autosomes. 97 single trait QTL were identified. After pleiotropy analysis the 97 loci coalesced into 35 pleiotropic QTLs. Cutoff for statistical significance is LPR=3.49but all pleiotropic loci listed in the reference are reported here.
On mouse Chromosome 8, Lbn7 (long bones 7) mapped to 29 cM near D8Mit293 (LPR=5.52). This locus is associated with necropsy body weight and ulna length. The confidence interval spans 0 cM- 32 cM. Previously identified QTL Adip4 (32 cM) may be identical to Lbn7. Potential candidate genes mapping near Lbn7 include Comp (33 cM), Spock3, Fkbp8, Zfp42 (24 cM), Irf2, Eif2s2 (*note: data in MGD places this gene on chromosome 2), and Fbxo8.
Bod5 (bone length and organs 5) mapped to 42 cM near D8Mit8 (LPR=7.03). This locus is associated with liver and kidney weights and femur, tibia, and humerus lengths. The confidence interval spans 22 cM - 52 cM. Potential candidate genes for Bod5 include Comp, Spock3, and Fkbp8.
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