Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  X
• Reference
  J:94605 Cheverud JM, et al., Quantitative trait loci for obesity- and diabetes-related traits and their dietary responses to high-fat feeding in LGXSM recombinant inbred mouse strains. Diabetes. 2004 Dec;53(12):3328-36
• ID
  MGI:3525656

Genes

Gene	Assay Type
Dbts2	visible phenotype
DXMit55	PCR amplified length variant
DXMit10	PCR amplified length variant
Gpr50	reported elsewhere
Avpr2	reported elsewhere
G6pdx	reported elsewhere
Pgr15l	reported elsewhere
Tsc22d3	reported elsewhere
Irs4	reported elsewhere
Capn6	reported elsewhere
Mbtps2	reported elsewhere
Obsty5	visible phenotype
DXMit121	PCR amplified length variant
DXMit186	PCR amplified length variant
Grpr	reported elsewhere
Ace2	reported elsewhere
Tlr8	reported elsewhere
Tlr7	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 16 LGXSM (LG=LG/J; SM=SM/J) recombinant inbred (RI) lines to identify QTLs associated with obesity and diabetes traits. Parental strain LG/J exhibits a larger body size compared to parental strain SM/J and these two strains also differ in obesity and diabetes related traits in response to dietary fat. 506 microsatellite markers at an average spacing of 2 to 3 cM were used in the genome scan. 39 loci were mapped but only those reaching genomewide significance are reported here.
  
  On mouse Chromosome X, an interval between DXMit55 (1.4 cM) and DXMit10 (63 cM) shows linkage to glucose tolerance at 20 weeks of age. This locus is named Dbts2 (diabetes 2). SM/J-derived alleles confer increased glucose tolerance at this locus. Dbts2 also shows suggestive linkage to fasting glucose at 20 weeks. Potential candidate genes mapping near Dbts2 are Gpr50 (26 cM), Avpr2 (29.5 cM), G6pdx (30 cM), Pgr15l, Tsc22d3, Irs4 (58 cM), Capn6 (58 cM), and Mbtps2.
  
  A second interval between DXMit121(67 cM) and DXMit186 (69 cM) shows linkage to total fat pad weight, mesenteric fat pad weight, leptin and cholesterol levels in female animals on a high fat diet. This locus is named Obsty5 (obesity 5). SM/J-derived alleles confer increased fat pad weights and increased leptin and cholesterol at Obsty5. Obsty5 also shows suggestive linkage to renal and reproductive fat pad weights in female animals on a high fat diet, body weight at necrosis, insulin levels, and spleen weight. Obsty5 also shows suggestive linkage to liver weight on a high fat diet. Potential candidate genes mapping near Obsty5 are Grpr (70 cM), Ace2 (70.5 cM), Tlr8, and Tlr7.