Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  15
• Reference
  J:56036 Southard-Smith EM, et al., Comparative analyses of the Dominant megacolon-SOX10 genomic interval in mouse and human. Mamm Genome. 1999 Jul;10(7):744-9
• ID
  MGI:1349703

Genes

Gene	Allele	Assay Type
D15Mit260		direct sequencing
H1f0		direct sequencing
Galr3		direct sequencing
Eif3l		direct sequencing
Polr2f		direct sequencing
Gcat		direct sequencing
Ankrd54		direct sequencing
Micall1		direct sequencing
Ankrd54		direct sequencing
Triobp		direct sequencing
Mprip		direct sequencing
1700088E04Rik		direct sequencing
Triobp		direct sequencing
EST974366		direct sequencing
Sox10	Sox10^Dom	direct sequencing

Notes

• Experiment
  The authors sequenced the BAC 43P19 to isentify the transcription units and regulatory sequences surronding the Sox10 interval. Approximately 170kb of sequence data was generated. this was compared with public databases to reveal matching sequences. Several genes and ESYs were identified. The order of these markers was not determined. The markers mapping to the Sox10 interval on mouse Chromosome 15 were: D15Mit260 - H1f0 - Galr3 - D15N1e - Polr2f - Gcat - EST1068184 - EST820961 - EST475269 - Triobp -Rhoip3 - ESTJ0827E04 - EST478828 - EST974366. It is unlikely that each of the ESTs represents a unique transcription unit. Further studies are on to determine the overlap of these ESTs within the transcription units in the interval. Comparison of this sequence with the sequence from the same interval on the syntenic human Chromosome 22 identified homologous human markers demonstrating conservation between the two species in the Dominant megacolon - Sox10 interval.