Gene Expression Image Detail

Reference

Figure

ID

J:149237 Perez-Balaguer A, et al., Mech Dev. 2009 May-Jun;126(5-6):301-13

7

MGI:6303977

Image

Caption

(A, B, D and E) Immunostaining at E12.5 and E18.5 in wild-type and conditional embryos. The arrow in (D) shows ectopic RN cells crossing the midline. The red nucleus is still specified in the absence of Shh, although, at E18.5 (B, E), it seems less compacted than in the wild-type. (C, F) Double labeled whole mount in situ hybridization at E12.5 of wild-type and conditional mutant embryo. The arrow in (F) indicates the loss of the mesencephalic Pax6 longitudinal positive band in the mutant. The arrowhead in (D, F) indicates the pretectal longitudinal band of Pax6 is not lost in the conditional mutant. The maintenance of the mesencephalic RN can be detected in the absence of Shh. (G, J) Immunostaining in transversal sections at E12.5. (H, I, K and L) RNA in situ hybridization of transversal sections. Abbreviations: RN, red nucleus; Di, diencephalon; Mb, midbrain.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.mod.2009.03.001 Mech Dev 126: 301-13, Perez-Balaguer A; Puelles E; Wurst W; Martinez S, Shh dependent and independent maintenance of basal midbrain. Copyright 2009.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:6304465 (Pou4f1)
B	MGI:6304465 (Pou4f1)
C	MGI:6304435 (Pax6) MGI:6304479 (Pou4f1)
D	MGI:6304465 (Pou4f1)
E	MGI:6304465 (Pou4f1)
F	MGI:6304435 (Pax6) MGI:6304479 (Pou4f1)
G	MGI:6304459 (Nkx6-1)
H	MGI:6304483 (Nkx6-2)
I	MGI:6304454 (Foxa2)
J	MGI:6304459 (Nkx6-1)
K	MGI:6304483 (Nkx6-2)
L	MGI:6304454 (Foxa2)