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Gene Expression Data
Assay Details
Assay
Reference: J:149237 Perez-Balaguer A, et al., Shh dependent and independent maintenance of basal midbrain. Mech Dev. 2009 May-Jun;126(5-6):301-13
Assay type: RNA in situ
MGI Accession ID: MGI:6304435
Gene symbol: Pax6
Gene name: paired box 6
Probe: Pax6 probe58
Probe preparation: Antisense RNA
Results
Specimen 3A: embryonic day 10.5 (more )
Note: Double labeled: blue - Pax6; red - Otx2 (assay MGI:6304436).
Structure Level Pattern Image Note
TS17: forebrain Present 3A Expression in the forebrain limits with the mid-forebrain border.
Specimen 3B: embryonic day 10.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: blue - Pax6; red - Otx2 (assay MGI:6304436).
Structure Level Pattern Image Note
TS17: forebrain Present 3B
Specimen 3C: embryonic day 12.5 (more )
Note: Double labeled: blue - Pax6; red - Otx2 (assay MGI:6304436).
Structure Level Pattern Image Note
TS20: forebrain Present 3C Expression in the forebrain limits with the mid-forebrain border.
TS20: midbrain lateral wall Present Regionally restricted 3C Expression was detected in a longitudinal band along the basal plate of the midbrain.
Specimen 3D: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: blue - Pax6; red - Otx2 (assay MGI:6304436).
Structure Level Pattern Image Note
TS20: forebrain Present 3D
TS20: midbrain lateral wall Absent 3D The longitudinal band along the basal plate of the midbrain was completely absent in the conditional mutant.
Specimen 7C: embryonic day 12.5 (more )
Note: Double labeled: red - Pax6; blue - Pou4f1 (assay MGI:6304479).
Structure Level Pattern Image Note
TS20: diencephalon lateral wall Present 7C There was a sharp expression boundary at the alar diencephalon and the alar midbrain. A band of expression was noted in the pretectum.
TS20: midbrain lateral wall Present Regionally restricted 7C A Pax6-positive stripe was noted in the basal plate.
Specimen 7F: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: red - Pax6; blue - Pou4f1 (assay MGI:6304479).
Structure Level Pattern Image Note
TS20: diencephalon lateral wall Present 7F The diencephalic band of expression remained in the conditional mutant.
TS20: midbrain lateral wall Absent 7F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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