Phenotype Image Detail

Image

Caption

RNA oxidation in Glul^tm1.1Geno/Glul^tm1.1Geno Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 mouse brain. (A-D) Survey of 8-OH(d)G immunoreactivity in brain tissue from Glul^tm1.1Geno/Glul^tm1.1Geno (Glul^fl/fl) (Left) and Glul^tm1.1Geno/Glul^tm1.1Geno Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 (Glul^fl/fl x Alb-Cre⁺) (Right) mice is presented in the absence (Lower) or presence (Upper) of costaining with GFAP (green) and Hoechst 34580 (blue). One representative of the cerebellum (A), hippocampus (B), somatosensory cortex (C), and piriform cortex (D) of n = 5 is demonstrated. (Scale bars: A, 100 um; B-D, 200 um.) (E) Samples harvested from brain sections (from left to right: cerebellum, hippocampus, somatosensory cortex, and piriform cortex) of Glul^tm1.1Geno/Glul^tm1.1Geno and Glul^tm1.1Geno/Glul^tm1.1Geno Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 mice were tested for RNA oxidation expression using Northwestern blotting [n = 6 for homozygous mice, n = 9 (cerebellum, hippocampus,and piriform cortex) and n = 10 (somatosensory cortex) for Glul^tm1.1Geno/Glul^tm1.1Geno Speer6-ps1^{Tg(Alb-cre)21Mgn}/0 mice.

Copyright

This image is from Qvartskhava N, Proc Natl Acad Sci U S A 2015 Apr 28;112(17):5521-6. Copyright 2015 National Academy of Sciences, U.S.A. J:221031

Associated Alleles

Symbol	Name
Glul^tm1.1Geno	glutamate-ammonia ligase; targeted mutation 1.1, Genoway
Speer6-ps1^Tg(Alb-cre)21Mgn	spermatogenesis associated glutamate (E)-rich protein 6, pseudogene 1; transgene insertion 21, Mark A Magnuson

Associated Genotypes

Allelic Composition	Genetic Background
Glul^tm1.1Geno/Glul^tm1.1Geno Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: C57BL/6 * C57BL/6J * DBA