Phenotype Image Detail

Image

Caption

Disruption of Mir10a leads to enhanced intestinal tumorigenesis in Apc^Min/Apc⁺ mice. (D) Left panel shows normal appearing small intestine with characteristic villi and well-ordered distribution of goblet cells together with basal location of epithelial nuclei. Middle panel is a typical example of a low-grade dysplasia in Apc^Min/Apc⁺ (WT) mice with accumulation of irregular goblet cells pattern (arrowheads) and some loss of nuclear polarity (indicated by "+"). Right panel shows a typical Apc^Min/Apc⁺ Mir10a^tm1.1Ahl/Mir10a^tm1.1Ahl (KO) high-grade dysplasia with a large area of loss of goblet cells, widespread loss of nuclear polarity, nuclear pleomorphism, and almost complete loss of villus organization (indicated by "*"). Note the transition from lower-grade dysplasia area with highly irregular goblet cell distribution (arrowheads). Scale bar = 100 uM. Whole intestines were paraffin-embedded as "Swiss rolls", sectioned and stained with hematoxylin and eosin. All animals were in a C57BL/6 background and between 110 and 160 days of age.

Copyright

This image is from Stadthagen G, PLoS Genet 2013 Oct;9(10):e1003913, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:205135

Associated Alleles

Symbol	Name
Apc^Min	APC, WNT signaling pathway regulator; multiple intestinal neoplasia
Mir10a^tm1.1Ahl	microRNA 10a; targeted mutation 1.1, Anders Lund

Associated Genotypes

Allelic Composition	Genetic Background
Apc^Min/Apc^+ Mir10a^tm1.1Ahl/Mir10a^tm1.1Ahl	B6.Cg-Mir10a^tm1.1Ahl Apc^min