Phenotype Image Detail

Image

Caption

Reduced internuclear commissure in Robo3-deficient mice. (A-F) Coronal section of P0 brains immuno-stained with Hb9 following Dil tracing of the VI-III internuclear connection. In controls (A and B), the internuclear commissure is strongly labeled (arrow), and Dil-labeled cells are observed at the level of the abducens nucleus (VI). (C and D) In Egr2^tm2(cre)Pch/Egr2⁺ Robo3^tm1.1Ache/Robo3^tm1.1Ache (Krox20::cre;Robo3^lox/lox) mutants, the Dil-labeled internuclear commissure is severely reduced. Some Dil-labeled fibers are still found on the contralateral side, but no cells are traced in the vicinity of the abducens nucleus. In Robo3^tm1Matl/Robo3^tm1Matl (Robo3^-/-) mice (E and F), the commissure is almost completely absent. No cells are traced on the contralateral side. Scale bars represent 100 um.

Copyright

This image is from Renier N, PLoS Biol 2010;8(3):e1000325, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:158918

Associated Alleles

Symbol	Name
Egr2^tm2(cre)Pch	early growth response 2; targeted mutation 2, Patrick Charnay
Robo3^tm1.1Ache	roundabout guidance receptor 3; targeted mutation 1.1, Alain Chedotal
Robo3^tm1Matl	roundabout guidance receptor 3; targeted mutation 1, Marc Tessier-Lavigne

Associated Genotypes

Allelic Composition	Genetic Background
Egr2^tm2(cre)Pch/Egr2^+ Robo3^tm1.1Ache/Robo3^tm1.1Ache	involves: 129S2/SvPas
Robo3^tm1Matl/Robo3^tm1Matl	Not Specified