Gene Expression Image Detail

Reference

Figure

ID

J:58233 Eisenstat DD, et al., J Comp Neurol. 1999 Nov 15;414(2):217-37

5

MGI:5522845

Image

Caption

Parasagittal sections. RNA-detection (blue-purple in cytoplasm) was followed by immunohistochemistry (brown-red in nucleus). Large photomicrographs (A,H,O) show representative views of the medial ganglionic eminence [ventricular zone (VZ) and subventricular zone (SVZ)]. Small photomicrographs are magnified views of selected regions from A, H, and O and other sections (not shown). The small photomicrographs show views of the VZ and SVZ from each in situ hybridization and immunohistochemistry double-labeling. A-G: Dlx-2 in situ hybridization followed by DLX-2 (B,C), DLX-1 (A,D,E), and DLX-5 (F,G) immunohistochemistry. H-N: Dlx-1 in situ hybridization followed by DLX-2 (H-J), DLX-1 (K,L), and DLX-5 (M,N) immunohistochemistry. O-U: Dlx-5 in situ hybridization followed by DLX-2 (O-Q), DLX-1 (R,S), and DLX-5 (T,U) immunohistochemistry. Asterisks indicate representative cells in the VZ (D,F,I) and the SVZ (N,Q,S) in which Dlx-2, Dlx-1, and/or Dlx-5 are coexpressed. Arrows in D and F mark the presence of Dlx-2 without detection of DLX-1 (D) or DLX-5 (F) in the VZ. Arrows in I and P demonstrate DLX-2 without coincident Dlx-1 (I) or Dlx-5 (P) expression. In M and R, arrows show VZ cells with Dlx-1 RNA(M) and DLX-1 protein (R) expression without DLX-5 (M) or Dlx-5 (R) expression, respectively. Arrowheads in O indicate the columnar arrangement of DLX-2-positive cells in the VZ. Double asterisks (A,H,O) indicate a transitional region between the VZ and the SVZ. Abbreviations: 1 Dlx-1 RNA; 2 Dlx-2 RNA; 5 Dlx-5 RNA; a1 DLX-1 immunoreactivity; a2 DLX-2 immunoreactivity; a5 DLX-5 immunoreactivity; AEP anterior entopeduncular area; AH anterior hypothalamus; BrdU bromodeoxyuridine; C cornea; CGE caudal ganglionic eminence; CX cortex; DT dorsal thalamus; EMT eminentia thalami; HCC hypothalamic cell cord; I first (diencephalic) Dlx domain; II second (telencephalic) Dlx domain; III third ventricle; LGE lateral ganglionic eminence; LT lamina terminalis; LV lateral ventricle; M M-phase; Me mesenchyme; MGE medial ganglionic eminence; MZ mantle zone; OE olfactory epithelium; OED optoeminential domain; ON optic nerve; OR optic recess; OV optic ventricle; p2-p6 prosomeres 2-6; PI propidium iodide; POA preoptic area; RCH retrochiasmatic area; RNE retinal neuroepithelium; S S-phase; SCH suprachiasmatic area; SE septum; Sp subpial; SPV supraoptic/paraventricular area; SVZ subventricular zone; VIT vitreous; VT ventral thalamus; VZ ventricular zone; ZLI zona limitans intrathalamica. Scale bars = 100 microns in A (also applies to H,O), 10 microns in G (also applies to B-F,I-N) and P (also applies to Q-U).

Copyright

This image is from Eisenstat DD, J Comp Neurol 1999 Nov 15;414(2):217-37, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:5523861 (Dlx1) MGI:5523240 (Dlx2)
B	MGI:5523056 (Dlx2) MGI:5523240 (Dlx2)
C	MGI:5523056 (Dlx2) MGI:5523240 (Dlx2)
D	MGI:5523861 (Dlx1) MGI:5523240 (Dlx2)
E	MGI:5523861 (Dlx1) MGI:5523240 (Dlx2)
F	MGI:5523240 (Dlx2) MGI:5523862 (Dlx5)
G	MGI:5523240 (Dlx2) MGI:5523862 (Dlx5)
H	MGI:5523863 (Dlx1) MGI:5523056 (Dlx2)
I	MGI:5523863 (Dlx1) MGI:5523056 (Dlx2)
J	MGI:5523863 (Dlx1) MGI:5523056 (Dlx2)
K	MGI:5523861 (Dlx1) MGI:5523863 (Dlx1)
L	MGI:5523861 (Dlx1) MGI:5523863 (Dlx1)
M	MGI:5523863 (Dlx1) MGI:5523862 (Dlx5)
N	MGI:5523863 (Dlx1) MGI:5523862 (Dlx5)
O	MGI:5523056 (Dlx2) MGI:5523864 (Dlx5)
P	MGI:5523056 (Dlx2) MGI:5523864 (Dlx5)
Q	MGI:5523056 (Dlx2) MGI:5523864 (Dlx5)
R	MGI:5523861 (Dlx1) MGI:5523864 (Dlx5)
S	MGI:5523861 (Dlx1) MGI:5523864 (Dlx5)
T	MGI:5523862 (Dlx5) MGI:5523864 (Dlx5)
U	MGI:5523862 (Dlx5) MGI:5523864 (Dlx5)