Phenotype Image Detail

Image

Caption

Nestin-Cre-mediated Trio deletion leads to developmental defects of the cerebellum. A-J', sagittal sections of cerebella at different developmental stages examined by Nissl staining (A-H') or hematoxylin and eosin staining (I-J'). A and B, at E16.5, control and Trio^tm1Mzhu/Trio^tm1Mzhu Tg(Nes-cre)1Kln/0 mutant (Trio^NKO) cerebella are relatively similar in size and structure. C-J', from P0.5, mutant cerebella are gradually smaller than control, and a clear IGL is not observed. The overall anteroposterior pattern of foliation is intact in the mutant, although several fissures forming sub-lobules (arrows in E and G) are not developed. Arrowheads represent principal fissures forming cardinal lobes, asterisks indicate fissures forming lobules, and arrows represent fissures forming sub-lobules. Roman numerals represent the corresponding lobules. G', H', and J' are magnifications of G, H, and J, respectively, emphasizing the lack of IGL in the mutant cerebella. Scale bars: A-H and I-J', 200 um; G' and H', 20 um.

Copyright

This image is from Peng YJ, J Biol Chem 2010 Aug 6;285(32):24834-44 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165902

Associated Alleles

Symbol	Name
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein
Trio^tm1Mzhu	triple functional domain (PTPRF interacting); targeted mutation 1, Min-Sheng Zhu

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Nes-cre)1Kln/0 Trio^tm1Mzhu/Trio^tm1Mzhu	involves: 129S6/SvEvTac * C57BL/6 * SJL