Phenotype Image Detail

Image

Caption

Angiogenesis in Tg(Upk2-TAg)1Rkl/0 (UPK2-SV40) mice with induced bladder carcinoma in situ. Angiogenesis was evaluated in the Tg(Upk2-TAg)1Rkl/0 mouse bladder after immunofluorescence labeling of frozen sections with CD31 or CD34 (green) and UPK II (red); the nuclei were stained with DAPI (blue). Representative microscopic images are shown (original magnification of 200x). A-D, increased vessels in SV40-derived bladder carcinoma in situ as shown by CD31 (A and C) and CD34 (B and D) immunofluorescence. E and F , immunofluorescence of frozen bladder samples from mutant and control mice showed a similar pericyte coverage (labeling for NG2, red) of tumor-associated vessels (labeling for CD31, green). G, quantification of CD31 immunofluorescence area, related to bladder area and normalized to control values, showed a statistically significant increase of vasculature in bladder from mutants. *, p = 0.017. H, pericyte coverage was quantified as the proportion of CD31+ vessels showing perivascular NG2 staining and was similar for both groups (0.17 in controls versus 0.15 in tumors; p = 0.511), thus reflecting a normal maturation of peritumoral vasculature in this stage of tumor development.

Copyright

This image is from Ayala de la Pena F, J Biol Chem 2011 Jun 10;286(23):20778-87 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:173505

Associated Alleles

Symbol	Name
Tg(Upk2-TAg)1Rkl	transgene insertion 1, Raghu Kalluri

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Upk2-TAg)1Rkl/0	involves: C57BL/6