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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8272302
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-NKX2-1 (ab76013)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen S2a WT: embryonic day 18.5 (more )
Note: Double labeled: red - Nkx2-1; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: globus pallidus Present S2a WT top
Specimen S2a Nolz1-/-: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Nkx2-1; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: globus pallidus Present S2a Nolz1-/- top
Specimen S2a Foxg1IresCre;Nolz1fl/fl: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Nkx2-1; green - Slc6a3 (assay MGI:8270447).
Structure Level Pattern Image Note
TS26: globus pallidus Present S2a Foxg1-IresCre;Nolz1fl/fl top
Specimen S2b Nolz1bgal/+: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503+ (more )
Note: Double labeled: red - Nkx2-1; green - Bcl11b (assay MGI:8270623).
Structure Level Pattern Image Note
TS26: globus pallidus Present S2b Nolz1bgal/+ top
Specimen S2b Nolz1bgal/bgal: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Nkx2-1; green - Bcl11b (assay MGI:8270623).
Structure Level Pattern Image Note
TS26: globus pallidus Present S2b Nolz1bgal/bgal top Expression in the globus pallidus was normal in the Nolz1<bgal/bgal> embryo.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory