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Gene Expression Data
Assay Details
Assay
Reference: J:290119 Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8270720
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-Caspase3 (Asp175) (D3E9) (#9579)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
Results
Specimen S1c WT: embryonic day 18.5 (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: midbrain Present S1c WT TH cCASP3 , S1c WT cCASP3 Apoptotic cells were immunopositive.
Specimen S1c Nolz1bGal/bGal: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: midbrain Present S1c Nolz1bgal/bgal cCASP3 , S1c Nolz1bgal/bgal TH cCASP3 Apoptotic cells were immunopositive.
Specimen S2d WT: embryonic day 18.5 (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: globus pallidus Present Regionally restricted S2d WT bottom , S2d WT top Apoptotic cells were immunopositive.
Specimen S2d Nolz1-/-: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: globus pallidus Present Regionally restricted S2d Nolz1-/- bottom , S2d Nolz1-/- top There was no increase in apoptosis in the mutant.
Specimen S2d Foxg1IresCre;Nolz1fl/fl: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: globus pallidus Present Regionally restricted S2d Foxg1-IresCre;Nolz1fl/fl bottom , S2d Foxg1-IresCre;Nolz1fl/fl top There was no increase in apoptosis in the mutant.
Specimen S6m: embryonic day 18.5 (more )
Note: Double labeled: red - Casp3; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: thalamus Ambiguous S6m
Specimen S6n: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Casp3; green - L1cam (assay MGI:8270624).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6n Within the thalamic region there was a strong increase in cleaved Casp3 expression in L1cam-labelled thalamic axons that project towards the striatum in Nolz1-/- mutant embryos.
Specimen S6o: embryonic day 18.5 (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: thalamus Ambiguous S6o
Specimen S6p: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6p There was a strong increase in cleaved Casp3 expression in Nolz1-/- mutant embryos, but not in other axonal populations.
Specimen S6q: embryonic day 18.5; Zfp503tm1.1Bpb/Zfp503tm1.1Bpb (more )
Note: Double labeled: red - Casp3; green - Zfp503 reporter (assay MGI:8273418).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6q , S6q' There was co-localization of cleaved Casp3 and beta-galactosidase reporter staining in the thalamus in Nolz1<bgal/bgal> embryos.
Specimen S6r: embryonic day 18.5 (more )
Note: Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: thalamus Ambiguous S6r
Specimen S6s: embryonic day 18.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Zfp503tm1.1Pnmn/Zfp503tm1.1Pnmn (more )
Note: Conditional mutant. Double labeled: red - Casp3; green - Th (assay MGI:8270717).
Structure Level Pattern Image Note
TS26: thalamus Present Regionally restricted S6s FoxG1-IRES-Cre;Nolz1<fl/fl> mutant embryos, which have an axon guidance phenotype, only show a minor induction of cleaved CASP3 in the thalamic region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory