Gene Expression Data
Assay Details
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Assay
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Reference:
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J:290119
Soleilhavoup C, et al., Nolz1 expression is required in dopaminergic axon guidance and striatal innervation. Nat Commun. 2020 Jun 19;11(1):3111
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:8273418
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Gene symbol:
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Zfp503
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Gene name:
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zinc finger protein 503
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase (4600-1409)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Nolz1-/- mutant embryos, in which the coding region of Nolz1 has been replaced by beta-Galactosidase (also referred to as Nolz1<bgal/bgal>). Zfp503<tm1.1Bpb> (neo cassette removed) has been used in the annotations, but it could be Zfp503<tm1Bpb> (neo cassette still present).
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Results
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Specimen
S6q:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Zfp503tm1.1Bpb/Zfp503tm1.1Bpb
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Zfp503 reporter; red - Casp3 (assay MGI:8270720).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: thalamus |
Present |
Regionally restricted |
S6q'
,
S6q
,
S6q''
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There was co-localization of cleaved Casp3 and beta-galactosidase reporter staining in the thalamus in Nolz1<bgal/bgal> embryos.
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Analysis Tools
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