Probe:	Mm-Lphn3 (317481)
Probe preparation:	Antisense DNA
Visualized with:	Fluorescence

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		4A Lphn1 2 3 , 4A Lphn3	Expression was detected in the cornu Ammonis and subiculum.
TS24: dentate gyrus	Present		4A Lphn1 2 3 , 4A Lphn3
TS24: entorhinal cortex	Present		4A Lphn1 2 3 , 4A Lphn3

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		4B Lphn1 2 3 , 4B Lphn3
TS27: entorhinal cortex	Present		4B Lphn1 2 3 , 4B Lphn3
TS27: hippocampus CA1	Present		4B Lphn1 2 3 , 4B Lphn3
TS27: hippocampus CA2	Present		4B Lphn1 2 3 , 4B Lphn3
TS27: hippocampus CA3	Present		4B Lphn1 2 3 , 4B Lphn3
TS27: subiculum	Present		4B Lphn1 2 3 , 4B Lphn3

Structure	Level	Pattern	Image	Note
TS28: hippocampus stratum oriens	Present	Regionally restricted	4E Lphn3 , s12_Lphn1 2 3 row 3 P10 , 4E Lphn1 2 3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10	Expression was detected in the stratum oriens of the CA1 and CA2 (weakly) regions.
TS28: CA1 pyramidal cell layer	Strong		4E Lphn1 2 3 , 4E Lphn3 , s12_Lphn1 2 3 Dapi row 3 P10 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 row 3 P10
TS28: CA2 pyramidal cell layer	Strong		s12_Lphn3 row 3 P10 , 4E Lphn3 , s12_Lphn1 2 3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn1 2 3
TS28: CA3 pyramidal cell layer	Present		s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn3 , s12_Lphn1 2 3 row 3 P10 , s12_Lphn3 row 3 P10 , 4E Lphn1 2 3
TS28: hippocampus stratum radiatum	Weak	Regionally restricted	4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 row 3 P10 , 4E Lphn1 2 3 , s12_Lphn1 2 3 Dapi row 3 P10	Weak expression was detected in the stratum radiatum of the CA3 region.
TS28: hippocampus stratum lacunosum	Absent		4E Lphn1 2 3 , s12_Lphn1 2 3 row 3 P10 , 4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10
TS28: dentate gyrus molecular layer	Absent		4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn1 2 3 , s12_Lphn1 2 3 row 3 P10
TS28: dentate gyrus granule cell layer	Present		4E Lphn3 , s12_Lphn1 2 3 row 3 P10 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn1 2 3
TS28: dentate gyrus polymorphic layer	Present		s12_Lphn1 2 3 row 3 P10 , 4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn1 2 3
TS28: subiculum	Present		4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , 4E Lphn1 2 3	Expression was detected in the presubiculum and subiculum.
TS28: entorhinal cortex	Present	Regionally restricted	4E Lphn3 , s12_Lphn3 row 3 P10 , s12_Lphn1 2 3 Dapi row 3 P10 , s12_Lphn1 2 3 row 3 P10 , 4E Lphn1 2 3	Essentially uniform expression was detected in layers 2-6 of the entorhinal cortex.

Structure	Level	Pattern	Image	Note
TS28: hippocampus stratum oriens	Present	Non-Uniform	s12_Lphn1 2 3 row 2 P30 , s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn3 row 2 P30 , 4F Lphn1 2 3 , 4F Lphn3
TS28: CA1 pyramidal cell layer	Strong		4F Lphn1 2 3 , s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn1 2 3 row 2 P30 , s12_Lphn3 row 2 P30 , 4F Lphn3
TS28: CA2 pyramidal cell layer	Present		s12_Lphn3 row 2 P30 , 4F Lphn3 , s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn1 2 3 row 2 P30 , 4F Lphn1 2 3
TS28: CA3 pyramidal cell layer	Present		s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn3 row 2 P30 , 4F Lphn1 2 3 , 4F Lphn3 , s12_Lphn1 2 3 row 2 P30
TS28: hippocampus stratum radiatum	Present	Non-Uniform	s12_Lphn1 2 3 row 2 P30 , s12_Lphn3 row 2 P30 , s12_Lphn1 2 3 Dapi row 2 P30 , 4F Lphn1 2 3 , 4F Lphn3
TS28: hippocampus stratum lacunosum	Weak	Regionally restricted	s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn1 2 3 row 2 P30 , 4F Lphn3 , s12_Lphn3 row 2 P30 , 4F Lphn1 2 3	Weak expression was observed in the stratum lacunosum of the CA2 region.
TS28: dentate gyrus molecular layer	Absent		4F Lphn1 2 3 , s12_Lphn3 row 2 P30 , s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn1 2 3 row 2 P30 , 4F Lphn3
TS28: dentate gyrus granule cell layer	Present		s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn1 2 3 row 2 P30 , s12_Lphn3 row 2 P30 , 4F Lphn1 2 3 , 4F Lphn3
TS28: dentate gyrus polymorphic layer	Present		s12_Lphn1 2 3 Dapi row 2 P30 , 4F Lphn1 2 3 , 4F Lphn3 , s12_Lphn1 2 3 row 2 P30 , s12_Lphn3 row 2 P30
TS28: subiculum	Present	Non-Uniform	s12_Lphn1 2 3 Dapi row 2 P30 , s12_Lphn3 row 2 P30 , 4F Lphn3 , s12_Lphn1 2 3 row 2 P30 , 4F Lphn1 2 3	A slight decrease was observed in the pre-subiculum.
TS28: entorhinal cortex	Present	Regionally restricted	s12_Lphn3 row 2 P30 , s12_Lphn1 2 3 row 2 P30 , 4F Lphn1 2 3 , s12_Lphn1 2 3 Dapi row 2 P30 , 4F Lphn3	Expression at a diminished level was detected in layers 2-6 of the entorhinal cortex.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		5E P10 red dotted 4 , 5E P10 left , 5E P10 red dotted 3	Higher levels of expression were detected in the CA1 stratum pyramidale.
TS28: CA2 pyramidal cell layer	Present		5E P10 left
TS28: CA3 pyramidal cell layer	Present		5E P10 left , 5E P10 blue dotted 4 , 5E P10 blue dotted 3
TS28: dentate gyrus granule cell layer	Present		5E P10 left
TS28: entorhinal cortex	Present		5E P10 left	There was no gradient in the expression.
TS28: subiculum	Present		5E P10 left	There was no gradient in the expression.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		5E P30 red dotted 4 , 5E P30 left , 5E P30 red dotted 3	Higher levels of expression were detected in the CA1 stratum pyramidale.
TS28: CA2 pyramidal cell layer	Present		5E P30 left
TS28: CA3 pyramidal cell layer	Present		5E P30 blue dotted 4 , 5E P30 left , 5E P30 blue dotted 3
TS28: dentate gyrus granule cell layer	Present		5E P30 left
TS28: entorhinal cortex	Present		5E P30 left	There was no gradient in the expression.
TS28: subiculum	Present		5E P30 left	There was no gradient in the expression.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6A Merge 1(D) , s13_Lphn3 row 1 P30 , s13_Lphn1 2 3 row 1 P30 , 6B 1(D) , s13_Lphn1 2 3 Dapi row 1 P30 , 6A Lphn3 1(D)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6A Lphn3 2 , s13_Lphn1 2 3 row 2 P30 , 6A Merge 2 , 6B 2 , s13_Lphn3 row 2 P30 , s13_Lphn1 2 3 Dapi row 2 P30

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		s13_Lphn1 2 3 row 3 P30 , 6A Lphn3 3 , s13_Lphn3 row 3 P30 , s13_Lphn1 2 3 Dapi row 3 P30 , 6A Merge 3 , 6B 3
TS28: entorhinal cortex	Present		6C Lphn3 top , 6A Merge 3 , 6B 3 , 6C Lphn1 2 3 top

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6A Merge 4(V) , s13_Lphn3 row 4 P30 , s13_Lphn1 2 3 row 4 P30 , s13_Lphn1 2 3 Dapi row 4 P30 , 6B 4(V) , 6A Lphn3 4(V)
TS28: entorhinal cortex	Present		6C Lphn1 2 3 bottom , 6C Lphn3 bottom , 6A Merge 4(V) , 6B 4(V)	In P30, the ventral-most caudomedial entorhinal cortex regions showed striking enrichment of Lphn3 in lower (L-V/VI) layers.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7B , 7B CA1 bottom , 7B'	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7B DG bottom , 7B , 7B''	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.
TS28: dentate gyrus polymorphic layer		Present	Regionally restricted	7B'' , 7B DG bottom , 7B	In the dentate gyrus polymorph layer excitatory hilar cells, Tenm3 and Lphn3 expression does not overlap.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7C , 7C' , 7C CA1 bottom	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7C , 7C'' , 7C DG bottom	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7F' , 7F , 7F CA1 middle	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7F , 7F'' , 7F DG middle	Tenms and Lphns expression largely overlapped in CA1 stratum pyramidale cells and dentate gyrus granule cells, albeit with varying levels of expression.

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s10_Lphn1 2 3 row 1 E16.5 , s10_Lphn3 row 1 E16.5 , s10_Lphn1 2 3 Dapi row 1 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s10_Lphn3 row 2 E16.5 , s10_Lphn1 2 3 Dapi row 2 E16.5 , s10_Lphn1 2 3 row 2 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s10_Lphn1 2 3 row 3 E16.5 , s10_Lphn1 2 3 Dapi row 3 E16.5 , s10_Lphn3 row 3 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s10_Lphn1 2 3 Dapi row 4 E16.5 , s10_Lphn3 row 4 E16.5 , s10_Lphn1 2 3 row 4 E16.5

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s10_Lphn1 2 3 Dapi row 1 P0 , s10_Lphn3 row 1 P0 , s10_Lphn1 2 3 row 1 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s10_Lphn1 2 3 row 2 P0 , s10_Lphn3 row 2 P0 , s10_Lphn1 2 3 Dapi row 2 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s10_Lphn1 2 3 row 3 P0 , s10_Lphn1 2 3 Dapi row 3 P0 , s10_Lphn3 row 3 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s10_Lphn1 2 3 Dapi row 4 P0 , s10_Lphn1 2 3 row 4 P0 , s10_Lphn3 row 4 P0

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s11_Lphn1 2 3 Dapi row 1 E16.5 , s11_Lphn3 row 1 E16.5 , s11_Lphn1 2 3 row 1 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s11_Lphn1 2 3 Dapi row 2 E16.5 , s11_Lphn3 row 2 E16.5 , s11_Lphn1 2 3 row 2 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s11_Lphn1 2 3 Dapi row 3 E16.5 , s11_Lphn3 row 3 E16.5 , s11_Lphn1 2 3 row 3 E16.5

Structure	Level	Pattern	Image	Note
TS24: hippocampal formation	Present		s11_Lphn1 2 3 row 4 E16.5 , s11_Lphn1 2 3 Dapi row 4 E16.5 , s11_Lphn3 row 4 E16.5

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s11_Lphn3 row 1 P0 , s11_Lphn1 2 3 Dapi row 1 P0 , s11_Lphn1 2 3 row 1 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s11_Lphn1 2 3 row 2 P0 , s11_Lphn3 row 2 P0 , s11_Lphn1 2 3 Dapi row 2 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s11_Lphn1 2 3 Dapi row 3 P0 , s11_Lphn3 row 3 P0 , s11_Lphn1 2 3 row 3 P0

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Present		s11_Lphn1 2 3 Dapi row 4 P0 , s11_Lphn1 2 3 row 4 P0 , s11_Lphn3 row 4 P0

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn1 2 3 row 1 P10 , s12_Lphn1 2 3 Dapi row 1 P10 , s12_Lphn3 row 1 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn1 2 3 row 2 P10 , s12_Lphn1 2 3 Dapi row 2 P10 , s12_Lphn3 row 2 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn1 2 3 Dapi row 4 P10 , s12_Lphn1 2 3 row 4 P10 , s12_Lphn3 row 4 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn1 2 3 row 1 P30 , s12_Lphn1 2 3 Dapi row 1 P30 , s12_Lphn3 row 1 P30

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn3 row 3 P30 , s12_Lphn1 2 3 row 3 P30 , s12_Lphn1 2 3 Dapi row 3 P30

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s12_Lphn1 2 3 Dapi row 4 P30 , s12_Lphn1 2 3 row 4 P30 , s12_Lphn3 row 4 P30

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s13_Lphn3 row 1 P10 , s13_Lphn1 2 3 Dapi row 1 P10 , s13_Lphn1 2 3 row 1 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s13_Lphn1 2 3 row 2 P10 , s13_Lphn1 2 3 Dapi row 2 P10 , s13_Lphn3 row 2 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s13_Lphn3 row 3 P10 , s13_Lphn1 2 3 Dapi row 3 P10 , s13_Lphn1 2 3 row 3 P10

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present		s13_Lphn3 row 4 P10 , s13_Lphn1 2 3 Dapi row 4 P10 , s13_Lphn1 2 3 row 4 P10