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Gene Expression Data
Assay Details
Assay
Reference: J:253351 Hanley O, et al., Parallel Pbx-Dependent Pathways Govern the Coalescence and Fate of Motor Columns. Neuron. 2016 Sep 7;91(5):1005-1020
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8206376
Gene symbol: Nos1
Gene name: nitric oxide synthase 1, neuronal
Results
Specimen 1C thoracic (Pbx3): embryonic day 11.5 (more )
Note: Double labeled: green - Nos1; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 1C Pbx3 nNos At thoracic levels, expression was detected in preganglionic motor column neurons, where it colocalized with Pbx3.
Specimen 2F Control: embryonic day 12.5 (more )
Note: Control animals are from age-matched littermates that are Cre-negative and either Pbx3+/+ or Pbx3+/-. Double labeled: green - Nos1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 2F Control Expression was in preganglionic motor column neurons.
Specimen 2F PbxMNdelta: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Conditional mutant. Double labeled: green - Nos1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Absent 2F PbxMNdelta
Specimen S2B Pbx3+/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3+ (more )
Note: Double labeled: green - Nos1; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B nNos Pbx3 Pbx3+/- Expression was in preganglionic motor column neurons.
Specimen S2B Pbx3-/-: embryonic day 12.5; Pbx3tm1Mlc/Pbx3tm1Mlc (more )
Note: Double labeled: green - Nos1; red - Pbx3 (assay MGI:8206356).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted S2B nNos Pbx3 Pbx3-/- Expression was normal in preganglionic motor column neurons in Pbx3-/- mice.
Specimen S2C: embryonic day 12.5; Olig2tm1(cre)Tmj/Olig2+, Pbx1tm1Koss/Pbx1tm1Koss, Pbx3tm1Mlc/Pbx3+ (more )
Note: Conditional mutant. Double labeled: green - Nos1; red - Isl1 (assay MGI:8206395) and Isl2 (assay MGI:8207778).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord thoracic region spinal cord motor neuron Weak Regionally restricted S2C nNos Isl1/2 Expression was reduced in preganglionic motor column neurons in Pbx1MNdelta,Pbx3+/- mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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