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Gene Expression Data
Assay Details
Assay
Reference: J:170604 Zhang Z, et al., The microRNA-processing enzyme Dicer is dispensable for somite segmentation but essential for limb bud positioning. Dev Biol. 2011 Mar 15;351(2):254-65
Assay type: RNA in situ
MGI Accession ID: MGI:8190643
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 probe
Assay notes: Tcre;Dicer or mutant samples could be either Tcre;Dicer<del/flox> or Tcre;Dicer<flox/flox>, which are phenotypically indistinguishable from one another. Tcre;Dicer<del/+> and Tcre;Dicer<flox/+> embryos are normal and used as controls.
Results
Specimen 2A: embryonic day 10.5; Dicer1tm1Bdh/Dicer1+, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: unsegmented mesenchyme Present 2A
Specimen 2B: embryonic day 10.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: unsegmented mesenchyme Present 2B Expression was detected in a normal pattern in the presomitic mesoderm region of mutant embryos at E10.5.
Specimen 5E: embryonic day 10.0; Dicer1tm1Bdh/Dicer1+, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: hindlimb bud Present 5E
Specimen 5F: embryonic day 10.0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: mesenchyme derived from lateral plate Absent 5F Lack of expression was observed in the mutant lateral plate mesoderm at the anterior-posterior position corresponding to the nascent hindlimb bud.
Specimen 5G: embryonic day 10.25; Dicer1tm1Bdh/Dicer1+, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: hindlimb bud Present 5G
Specimen 5H: embryonic day 10.25; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(T-cre)1Lwd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: hindlimb bud Present Regionally restricted 5H Expression was later and in a more posterior position in the mutant compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory