Probe:	Pax6 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		2B
TS21: lateral ganglionic eminence ventricular zone	Strong	Regionally restricted	2B	High level expression in a small ventricular territory only a few cell diameters wide (pLGE1). Expression in the rest region (pLGE2-4) was in a lower level.
TS21: medial ganglionic eminence	Absent		2B	Also no expression in the septum.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		3B
TS21: lateral ganglionic eminence ventricular zone	Strong	Regionally restricted	3B	High level expression in a small ventricular territory only a few cell diameters wide (pLGE1). Low expression in the pLGE2-3.
TS21: thalamus	Present		3B
TS21: diencephalon	Strong	Regionally restricted	3B	Expressed in the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		4J , 4F	Expressed in pLGE1, pLGE2, and pLGE3.
TS21: diencephalon	Present	Regionally restricted	4J , 4F	Expressed in the eminentia thalami.
TS21: prethalamus	Present		4J , 4F
TS21: thalamus	Present		4F
TS21: hypothalamus	Present	Regionally restricted	4J , 4F	Expressed in the progenitor domain from the hypothalamic preoptic area.
TS21: brain nucleus	Present	Regionally restricted	4F	Expressed in the supraoptic paraventricular region.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present		5C
TS21: thalamus	Present		5C
TS21: cerebral cortex ventricular layer	Strong		5C
TS21: prethalamus	Strong		5C
TS21: diencephalon	Present		5C	Expressed in the eminentia thalami.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		6B
TS21: lateral ganglionic eminence	Present		6B
TS21: telencephalon	Present	Regionally restricted	6B	Expressed in the progenitor domains of the septum (pSe1, pSe2, and pSe3).

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	S1B	Expressed at high levels in the pallial ventricular zone and lateral ganglionic eminence progenitor ventricular region 1 (pLGE1), at low levels in pLGE2, pLGE3 and pLGE4, and was not expressed in the medial ganglionic eminence ventricular zone.