Gene Expression Data
Assay Details
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Assay
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Reference:
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J:297650
Roeseler DA, et al., Gbx2 is required for the migration and survival of a subpopulation of trigeminal cranial neural crest cells. J Dev Biol. 2020;8(33)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7840239
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Gene symbol:
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Sox10
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Gene name:
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SRY (sex determining region Y)-box 10
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Probe:
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Sox10 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A:
embryonic day 9.5
(more  )
Note: Genetic background of specimen was either wild type or heterozygous.
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Genetic background of specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: migrating neural crest |
Present |
Regionally restricted |
3A
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Expression in neural crest cells migrating from rhombomere 2 into 1st branchial arch. Expression in trigeminal cranial neural crest cells and hyoid cranial neural crest cell population.
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| TS15: otocyst |
Present |
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3A
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Gbx2tm1Mrt/Gbx2tm1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: migrating neural crest |
Present |
Regionally restricted |
3B
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Trigeminal cranial neural crest cells fail to migrate into 1st branchial arch and remain adjacent to dorsal neural tube, abnormally expressed as a lateral stripe. Expression in hyoid cranial neural crest cell population unaffected compared to wild type.
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| TS15: otocyst |
Present |
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3B
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Expression in otic vesicle unaffected compared to wild type.
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Specimen
3C:
embryonic day 9.5
(more )
Note: Genetic background of specimen was either wild type or heterozygous.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Genetic background of specimen was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: migrating neural crest |
Present |
Regionally restricted |
3C
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Expression in neural crest cells migrating from rhombomere 2 into 1st branchial arch. Expression in trigeminal cranial neural crest cells and hyoid cranial neural crest cell population
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| TS15: otocyst |
Present |
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3C
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Gbx2tm1Mrt/Gbx2tm1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: migrating neural crest |
Present |
Regionally restricted |
3D
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Trigeminal cranial neural crest cells fail to migrate into 1st branchial arch and remain adjacent to dorsal neural tube. Expression in hyoid cranial neural crest cell population unaffected compared to wild type.
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| TS15: rhombomere 2 |
Present |
Punctate |
3D
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Additional punctate expression in dorsal neuroepithelium of rhombomere 2.
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| TS15: otocyst |
Present |
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3D
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Expression in otic vesicle unaffected compared to wild type.
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Specimen
3E:
embryonic day 9.5
(more )
Note: Genetic background of specimen was either wild type or heterozygous. Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
Specimen
3E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Genetic background of specimen was either wild type or heterozygous. Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Gbx2tm1Mrt/Gbx2tm1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
3F
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Expression is anterior to that of Egr2.
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Specimen
3G:
embryonic day 9.5
(more )
Note: Genetic background of specimen was either wild type or heterozygous. Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
Specimen
3G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Genetic background of specimen was either wild type or heterozygous. Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Mutant Allele(s):
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Gbx2tm1Mrt/Gbx2tm1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Double labeled: blue - Sox10; orange - Egr2 (assay MGI:7840234).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
3H
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Expression is anterior to that of Egr2.
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