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Gene Expression Data
Assay Details
Assay
Reference: J:75859 Wigle JT, et al., An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype. EMBO J. 2002 Apr 2;21(7):1505-13
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7713272
Gene symbol: Prox1
Gene name: prospero homeobox 1
Reporter: lacZ
Antibody: anti-B-galactosidase
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 12.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
Structure Cell Type Level Pattern Image Note
TS20: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 2A Co-expression with Pecam1 in budding lymphatic endothelial cells.rr
Specimen 2B: embryonic day 12.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
Structure Cell Type Level Pattern Image Note
TS20: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 2B Co-expression with Flt4 in budding lymphatic endothelial cells.
Specimen 3A: embryonic day 14.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
Structure Cell Type Level Pattern Image Note
TS22: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 3A Co-expression with Pecam1 in budding lymphatic endothelial cellss, interspersed with blood vessels and capillaries.
Specimen 3A': embryonic day 14.5; Prox1tm1Gco/Prox1tm1Gco (more )
Note: Double labeled: green - Prox1 reporter; red - Pecam1 (assay MGI:7713283).
Structure Level Pattern Image Note
TS22: thoracic body wall Absent 3A' Authors report absence of lymphatic vasculature.
Specimen 3B: embryonic day 14.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
Structure Cell Type Level Pattern Image Note
TS22: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 3B Co-expression with Flt4 in budding lymphatic endothelial cells, interspersed with blood vessels and capillaries.
Specimen 3B': embryonic day 14.5; Prox1tm1Gco/Prox1tm1Gco (more )
Note: Double labeled: green - Prox1 reporter; red - Flt4 (assay MGI:7713281).
Structure Level Pattern Image Note
TS22: thoracic body wall Absent 3B' Authors report absence of lymphatic vasculature.
Specimen 6C: embryonic day 11.5; Prox1tm1Gco/Prox1+ (more )
Structure Level Pattern Image Note
TS19: thoracic body wall Present Regionally restricted 6C Expression adjacent to cardinal vein.
Specimen 6D: embryonic day 11.5; Prox1tm1Gco/Prox1tm1Gco (more )
Structure Level Pattern Image Note
TS19: thoracic body wall Present Regionally restricted 6D Expression adjacent to cardinal vein, in fewer cells than in heterozygote.
Specimen 7A: embryonic day 11.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Lyve1 (assay MGI:7713282).
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7A Co-expression with Lyve1 in lymphatic endothelial cells.
Specimen 7B: embryonic day 11.5; Prox1tm1Gco/Prox1tm1Gco (more )
Note: Double labeled: green - Prox1 reporter; red - Lyve1 (assay MGI:7713282).
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7B Expression in lymphatic endothelial cells. No co-expression with Lyve1.
Specimen 7C: embryonic day 11.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1; red - laminin.
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7C Expression in lymphatic endothelial cells.
Specimen 7D: embryonic day 11.5; Prox1tm1Gco/Prox1tm1Gco (more )
Note: Double labeled: green - Prox1; red - laminin.
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7D Expression in lymphatic endothelial cells.
Specimen 7E: embryonic day 11.5; Prox1tm1Gco/Prox1+ (more )
Note: Double labeled: green - Prox1 reporter; red - Cd34 (assay MGI:7713285).
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7E Expression in lymphatic endothelial cells.
Specimen 7F: embryonic day 11.5; Prox1tm1Gco/Prox1tm1Gco (more )
Note: Double labeled: green - Prox1 reporter; red - Cd34 (assay MGI:7713285).
Structure Cell Type Level Pattern Image Note
TS19: lymphatic system endothelial cell of lymphatic vessel Present Regionally restricted 7F Expression in lymphatic endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
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