Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75859
Wigle JT, et al., An essential role for Prox1 in the induction of the lymphatic endothelial cell phenotype. EMBO J. 2002 Apr 2;21(7):1505-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7713282
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Gene symbol:
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Lyve1
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Gene name:
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lymphatic vessel endothelial hyaluronan receptor 1
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Antibody:
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Anti-LYVE-1
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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Two Lyve1 antibodies were described, but the one used in this assay was not detailed. Anti-LYVE-1MGI:7713252 may have been used in this assay.
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Results
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Specimen
1D:
embryonic day 10.5; Prox1tm1Gco/Prox1 +
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: cardinal vein |
vein endothelial cell |
Present |
Regionally restricted |
1D
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Uniform expression in endothelial cells lining cardinal vein.
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| TS17: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
1D
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Expression in budding lymphatic endothelial cells.
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Specimen
2C:
embryonic day 12.5; Prox1tm1Gco/Prox1 +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
2C
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Expression restricted to budding lymphatic endothelial cells.
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Specimen
3C:
embryonic day 14.5; Prox1tm1Gco/Prox1 +
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
3C
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Expression overlapping with Prox1 reporter expression in lymphatic endothelial cells.
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| TS22: vascular element |
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Present |
Regionally restricted |
3C
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Expression in capillary-like structures.
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| TS22: thoracic body wall |
macrophage |
Present |
Scattered |
3C
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Expression only in scattered macrophages.
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Specimen
3C':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: thoracic body wall |
macrophage |
Present |
Scattered |
3C'
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Expression only in scattered macrophages.
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Specimen
6G:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: cardinal vein |
vein endothelial cell |
Weak |
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6G
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Weak expression in endothelial cells lining cardinal vein.
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| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Strong |
Regionally restricted |
6G
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Strong expression in budding lymphatic endothelial cells.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: cardinal vein |
vein endothelial cell |
Present |
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6H
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Expression restricted to endothelial cells of cardinal vein.
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Specimen
7A:
embryonic day 11.5; Prox1tm1Gco/Prox1 +
(more )
Note: Double labeled: red - Lyve1; green - Prox1 reporter (assay MGI:7713272).
Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Prox1 reporter (assay MGI:7713272).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Present |
Regionally restricted |
7A
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Co-expression with Prox1 in lymphatic endothelial cells.
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| TS19: thoracic body wall |
macrophage |
Present |
Scattered |
7A
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Expression in scattered macrophages.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Prox1tm1Gco/Prox1tm1Gco
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Lyve1; green - Prox1 reporter (assay MGI:7713272).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: lymphatic system |
endothelial cell of lymphatic vessel |
Absent |
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7B
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No expression in lymphatic endothelial cells.
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