Probe:	MTF#275
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS27: suprachiasmatic nucleus	Present	Regionally restricted	1_Lhx1	Regional-specific expression was detected in the suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS28: suprachiasmatic nucleus	Present	Regionally restricted	2A Lhx1	Regional-specific expression was detected in the suprachiasmatic nucleus. Expression covered 52% of the central suprachiasmatic nucleus and partially overlapped both ventrolateral/core and dorsomedial/shell regions.

Structure	Level	Pattern	Image	Note
TS28: suprachiasmatic nucleus	Present	Regionally restricted		Expression was identical to that seen at P15.

Structure	Level	Pattern	Image	Note
TS28: suprachiasmatic nucleus	Present	Regionally restricted		Expression was identical to that seen at P15.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4_Lhx1 E14.5	Expression was present in discrete bilateral regions distal to the third ventricle within the broader Six3 domain.

Structure	Level	Pattern	Image	Note
TS23: hypothalamus	Present	Regionally restricted	4_Lhx1 E15.5	An increase in the area and approximate number of expressing cells was observed at E15.5.

Structure	Level	Pattern	Image	Note
TS25: suprachiasmatic nucleus	Present	Regionally restricted	4_Lhx1 E17.5	Expression was reduced at E17.5 to a central portion of the suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS27: suprachiasmatic nucleus	Present	Regionally restricted	4_Lhx1 P0	Expression remained centralized in the suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS17: diencephalon floor plate	Ambiguous	Regionally restricted	6A E10.5 Lhx1	Expression wasn't described in the suprachiasmatic progenitor domain at E10.5.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6A E11.5 Lhx1	The onset of expression at E11.5 was observed in the most ventral aspects of tissue beneath the third ventricle. Expression was in a very small band of cells at the ventral edge of the Six3-expressing zone.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	6A E12.5 Lhx1	Both a dorsal and rostrocaudal expansion in the number of cells expressing Lhx1 occurred from E12.5 to E13.5.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6A E13.5 Lhx1	Both a dorsal and rostrocaudal expansion in the number of cells expressing Lhx1 occurred from E12.5 to E13.5.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Ambiguous		6D E11.5 Rostral 1 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	6D E11.5 2 Lhx1	Expression was in a very small band of cells at the ventral edge of the Six3-expressing zone.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Ambiguous		6D E11.5 3 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Ambiguous		6D E11.5 4 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Ambiguous		6D E11.5 Caudal 5 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Ambiguous		6D E13.5 Rostral 1 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 2 Lhx1	A rostrocaudal expansion in the number of cells expressing Lhx1 occurred.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 3 Lhx1	A rostrocaudal expansion in the number of cells expressing Lhx1 occurred.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Present	Regionally restricted	6D E13.5 4 Lhx1	A rostrocaudal expansion in the number of cells expressing Lhx1 occurred.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Ambiguous		6D E13.5 Caudal 5 Lhx1	Expression was detected in discrete zones along the rostrocaudal axis.

Structure	Level	Pattern	Image	Note
TS23: hypothalamus medial zone	Present	Regionally restricted	8C top Lhx1	Expression was retained in the early suprachiasmatic nucleus unilaterally in mice displaying partial Six3 deletion. Loss of expression was only observed in regions lacking Six3.

Structure	Level	Pattern	Image	Note
TS23: hypothalamus medial zone	Present	Regionally restricted	8C bottom Lhx1	Mutant mice that retained expression of Six3 showed prominent expression in the early postmitotic suprachiasmatic nucleus.