Gene Expression Data
Assay Details
Assay
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Reference:
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J:171421
Vandunk C, et al., Development, maturation, and necessity of transcription factors in the mouse suprachiasmatic nucleus. J Neurosci. 2011 Apr 27;31(17):6457-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7660840
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Gene symbol:
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Six6
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Gene name:
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sine oculis-related homeobox 6
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Probe:
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MTF#179
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Three possible probes were referenced: MGI:3506049, MGI:3506055 and MGI:3506054. The probe used in this assay was not specified.
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|
Results
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Specimen
1:
postnatal day 0
(more )
Specimen
1:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
6B E10.5:
embryonic day 10.5
(more )
Specimen
6B E10.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6B E11.5:
embryonic day 11.5
(more )
Specimen
6B E11.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6B E12.5:
embryonic day 12.5
(more )
Specimen
6B E12.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6B E13.5:
embryonic day 13.5
(more )
Specimen
6B E13.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6D E11.5 Rostral 1:
embryonic day 11.5
(more )
Note: Rostral-most section of a rostral-caudal series beginning from rostral eye fields level.
Specimen
6D E11.5 Rostral 1:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Rostral-most section of a rostral-caudal series beginning from rostral eye fields level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
6D E11.5 Rostral 1 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
TS19: telencephalon |
Present |
Regionally restricted |
6D E11.5 Rostral 1 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
Specimen
6D E11.5 2:
embryonic day 11.5
(more )
Note: Second section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
Specimen
6D E11.5 2:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Second section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
6D E11.5 2 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
TS19: telencephalon |
Present |
Regionally restricted |
6D E11.5 2 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
Specimen
6D E11.5 3:
embryonic day 11.5
(more )
Note: Third section of a rostral-caudal series.
Specimen
6D E11.5 3:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Third section of a rostral-caudal series.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
6D E11.5 3 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
TS19: telencephalon |
Present |
Regionally restricted |
6D E11.5 3 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
Specimen
6D E11.5 4:
embryonic day 11.5
(more )
Note: Fourth section of a rostral-caudal series.
Specimen
6D E11.5 4:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fourth section of a rostral-caudal series.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
6D E11.5 4 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
TS19: telencephalon |
Present |
Regionally restricted |
6D E11.5 4 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
Specimen
6D E11.5 Caudal 5:
embryonic day 11.5
(more )
Note: Caudal-most section of a rostral-caudal series ending at developing pituitary.
Specimen
6D E11.5 Caudal 5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Caudal-most section of a rostral-caudal series ending at developing pituitary.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
6D E11.5 Caudal 5 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
TS19: telencephalon |
Present |
Regionally restricted |
6D E11.5 Caudal 5 Six6
|
Expression was first seen at E11.5 in a ventrally restricted pattern within the entire rostrocaudal region examined.
|
Specimen
6D E13.5 Rostral 1:
embryonic day 13.5
(more )
Note: Rostral-most section of a rostral-caudal series beginning from rostral eye fields level.
Specimen
6D E13.5 Rostral 1:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Rostral-most section of a rostral-caudal series beginning from rostral eye fields level.
|
Specimen
6D E13.5 2:
embryonic day 13.5
(more )
Note: Second section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
Specimen
6D E13.5 2:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Second section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: diencephalon |
Present |
Regionally restricted |
6D E13.5 2 Six6
|
Expression was consistent along the rostrocaudal ventral surface.
|
Specimen
6D E13.5 3:
embryonic day 13.5
(more )
Note: Third section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
Specimen
6D E13.5 3:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Third section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: diencephalon |
Present |
Regionally restricted |
6D E13.5 3 Six6
|
Expression was consistent along the rostrocaudal ventral surface.
|
Specimen
6D E13.5 4:
embryonic day 13.5
(more )
Note: Fourth section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
Specimen
6D E13.5 4:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fourth section of a rostral-caudal series. This section contained the suprachiasmatic nucleus progenitor region.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: diencephalon |
Present |
Regionally restricted |
6D E13.5 4 Six6
|
Expression was consistent along the rostrocaudal ventral surface.
|
Specimen
6D E13.5 Caudal 5:
embryonic day 13.5
(more )
Note: Caudal-most section of a rostral-caudal series ending at developing pituitary.
Specimen
6D E13.5 Caudal 5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Caudal-most section of a rostral-caudal series ending at developing pituitary.
|
|