Probe:	Hoxd12 probe
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3D E10.5

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	3D E11.0	Expression was detected in the proximal-posterior region, corresponding to the posterior zeugopod.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	3D E11.5	Expression was detected in the proximal-posterior region, corresponding to the posterior zeugopod.

Structure	Level	Pattern	Image	Note
TS20: autopod	Present		3D E12.0
TS20: zeugopod	Present	Regionally restricted	3D E12.0	Expression in the posterior zeugopod was weaker than in the autopod.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		3H E10.5

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	3H E11.0	Expression was detected in the proximal-posterior region, corresponding to the posterior zeugopod.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	3H E11.5	Expression was detected in the proximal-posterior region, corresponding to the posterior zeugopod.

Structure	Level	Pattern	Image	Note
TS20: autopod	Present		3H E12.0
TS20: zeugopod	Present	Regionally restricted	3H E12.0	Expression in the posterior zeugopod was weaker than in the autopod.
TS20: hindlimb pre-cartilage condensation	Present	Regionally restricted	3H E12.0	Expression was detected during cartilage formation of the fibula.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	6D E11.0 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Weak	Regionally restricted	6D E11.0 Zeugopod	Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud.

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Widespread	6D E11.5 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak	Regionally restricted	6D E11.5 Zeugopod	Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud.

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Widespread	6D E12.0 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS20: forearm	Absent		6D E12.0 Zeugopod
TS20: forelimb	Present	Regionally restricted	6D E12.0 Zeugopod	A thin layer of expression was seen in the posterior periphery of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	6H E11.0 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Weak	Regionally restricted	6H E11.0 Zeugopod	Expression in the zeugopod was relatively weak but had a domain in the posterior portion with a clear boundary at the middle of the limb bud. Expression was detected in the presumptive fibula region.

Structure	Level	Pattern	Image	Note
TS19: footplate	Present	Widespread	6H E11.5 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	6H E11.5 Zeugopod , 8A Mouse hindlim Hoxd12	Expression in the hindlimb zeugopod was decreased at E11.5

Structure	Level	Pattern	Image	Note
TS20: foot	Present	Widespread	6H E12.0 Autopod	Wide expression was detected in the autopod, expect in a small, anterior portion.

Structure	Level	Pattern	Image	Note
TS20: lower leg	Absent		6H E12.0 Zeugopod
TS20: hindlimb	Present	Regionally restricted	6H E12.0 Zeugopod	A thin layer of expression was seen in the posterior periphery of the hindlimb bud.