Probe:	Timp2 probe
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Cell Type	Level	Pattern	Image	Note
TS28: mesenchymal stroma of ovary		Present	Diffuse	2B , 2A	Diffuse expression in stroma surrounding follicles.
TS28: ovary follicle cell	granulosa cell	Absent		2A , 2B	Expression absent in granulosa cells of follicles.

Structure	Level	Pattern	Image	Note
TS28: mesometrium	Present		2E , 2D	Expression predominantly present in parametrium and myometrium.
TS28: myometrium	Present		2E , 2D	Expression predominantly present in parametrium and myometrium.
TS28: endometrium glandular epithelium	Absent		2D , 2E
TS28: endometrium luminal epithelium	Absent		2D , 2E

Structure	Level	Pattern	Image	Note
TS28: muscle layer of epididymis	Strong		2G , 2F	Strong expression in capsule and in smooth muscle cells surrounding ductus epididymis.
TS28: epithelium of epididymis	Absent		2G , 2F	Expression absent in tubular epithelium.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Strong		2H	Strong expression restricted to interstitial Leydig cells.
TS28: testis epithelium		Absent		2H	Expression absent in germinal epithelium.
TS28: Sertoli cell	Sertoli cell	Absent		2H

Structure	Level	Pattern	Image	Note
TS28: femoral artery	Present	Regionally restricted	3B , 3A	Expression in adventitial and medial layers of artery.
TS28: femoral vein	Trace		3B , 3A	Little expression in vein.
TS28: striated muscle tissue	Absent		3A , 3B	No expression in striated muscle cells around blood vessels.`

Structure	Level	Pattern	Image	Note
TS28: pericardium	Present	Regionally restricted	3C , 3D	Expression restricted to pericardium, in particular around the auricle.
TS28: cardiac muscle tissue	Absent		3D , 3C	No expression in myocardium and endocardium.
TS28: endocardial lining	Absent		3D , 3C	No expression in myocardium and endocardium.

Structure	Level	Pattern	Image	Note
TS28: alveolar septum	Present		3E , 3F	Expression in pleura (mesothelium) and interalveolar septa.
TS28: pleura	Present		3E , 3F	Expression in pleura (mesothelium) and interalveolar septa.

Structure	Level	Pattern	Image	Note
TS28: lung connective tissue	Present		3G	Expression in connective tissue surrounding airways.
TS28: bronchiole epithelium	Absent		3G	No expression in cuboidal epithelium of bronchus or respiratory bronchioles.
TS28: lobar bronchus epithelium	Absent		3G	No expression in cuboidal epithelium of bronchus or respiratory bronchioles.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle choroid plexus	Present		4A	Expression in meninges, choroid plexus, and neural tissues of neocortex, septum, and piriform olfactory cortex.
TS28: meninges	Present		4A	Expression in meninges, choroid plexus, and neural tissues of neocortex, septum, and piriform olfactory cortex.
TS28: neocortex	Present		4A	Expression in meninges, choroid plexus, and neural tissues of neocortex, septum, and piriform olfactory cortex.
TS28: piriform cortex	Present		4A	Expression in meninges, choroid plexus, and neural tissues of neocortex, septum, and piriform olfactory cortex.
TS28: septal nucleus	Present		4A	Expression in meninges, choroid plexus, and neural tissues of neocortex, septum, and piriform olfactory cortex.
TS28: corpus callosum	Absent		4A
TS28: corpus striatum	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		4B

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle choroid plexus	Strong		4D , 4C	Expression particularly intense in invaginated folds of pia mater forming choroid plexus.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		4E , 4F	Expression specifically expressed in 2nd (external granular) and 4th (internal granular) layers containing neurons and glial cells.
TS28: cortical layer IV	Present		4E , 4F	Expression specifically expressed in 2nd (external granular) and 4th (internal granular) layers containing neurons and glial cells.
TS28: meninges	Present		4E , 4F	Expression specifically expressed in 2nd (external granular) and 4th (internal granular) layers containing neurons and glial cells.

Structure	Level	Pattern	Image	Note
TS28: dermis connective tissue	Present		5A , 5B	Expression in connective tissue cells of dermis and around hair follicles.
TS28: epidermis	Absent		5B , 5A
TS28: skin	Present	Regionally restricted	5B , 5A	No expression in subcutaneous tissue.

Structure	Level	Pattern	Image	Note
TS28: intestinal villus	Absent		5C , 5D	No expression in epithelium of villi or crypt or in muscularis mucosae.
TS28: small intestine crypt of lieberkuhn	Absent		5C , 5D	No expression in epithelium of villi or crypt or in muscularis mucosae.
TS28: small intestine muscularis mucosa	Absent		5C , 5D	No expression in epithelium of villi or crypt or in muscularis mucosae.

Structure	Level	Pattern	Image	Note
TS28: liver parenchyma	Absent		5E , 5F	No expression in parenchyma, central vein or portal space.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		7A	Expression mainly in decidua.

Structure	Level	Pattern	Image	Note
TS19: junctional zone	Weak		7A	Lower expression in junctional zone.

Structure	Level	Pattern	Image	Note
TS28: decidua	Weak		7B	Relative decrease in expression compared to E11.5.

Structure	Level	Pattern	Image	Note
TS25: junctional zone	Strong		7B	Expression dramatically increased in junctional zone.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	7E , 7D	Expression confined to cells surrounding central artery and materanl blood vessels.

Structure	Level	Pattern	Image	Note
TS25: junctional zone	Present	Regionally restricted	7F , 7G	Expression in junctional zone separating labyrinth from zone of giant cells.
TS25: yolk sac	Absent		7F , 7G

Structure	Level	Pattern	Image	Note
TS25: spongiotrophoblast	Absent		7H , 7I	Intensive expression in spongiotrophoblasts.
TS25: trophoblast giant cell	Absent		7H , 7I

Structure	Level	Pattern	Image	Note
TS20: brain ventricle	Present		8A	Intense expression in snout, oropharynx, nasopharynx, tongue, diaphragm, bladder, spine and brain vesicles.
TS20: diaphragm	Present		8A	Intense expression in snout, oropharynx, nasopharynx, tongue, diaphragm, bladder, spine and brain vesicles.
TS20: nasopharynx	Present		8A	Intense expression in snout, oropharynx, nasopharynx, tongue, diaphragm, bladder, spine and brain vesicles.
TS20: oropharynx	Present		8A	Intense expression in snout, oropharynx, nasopharynx, tongue, diaphragm, bladder, spine and brain vesicles.
TS20: tongue	Present		8A	Intense expression in snout, oropharynx, nasopharynx, tongue, diaphragm, bladder, spine and brain vesicles.

Structure	Level	Pattern	Image	Note
TS23: head mesenchyme	Present	Regionally restricted	8B , 8C	In the snout, abundant expression in connective tissues surrounding nasal and oral epithelia.

Structure	Level	Pattern	Image	Note
TS22: secondary palate	Present		8D	Intense expression in forming palate and tongue muscle.
TS22: tongue muscle	Present		8D	Intense expression in forming palate and tongue muscle.
TS22: head mesenchyme	Present	Regionally restricted	8D	Intense expression in connective tissue surrounding nasal, oropharyngeal, laryngeal and esophageal epithelia.
TS22: esophagus epithelium	Absent		8D	No expression in nasal, orophyangeal, laryngeal and esophageal epithelia.
TS22: nasal epithelium	Absent		8D	No expression in nasal, orophyangeal, laryngeal and esophageal epithelia.
TS22: oropharynx	Absent		8D	No expression in nasal, orophyangeal, laryngeal and esophageal epithelia.
TS22: pituitary gland	Absent		8D	No expression in thymus and hypophyseal gland
TS22: thymus primordium	Absent		8D	No expression in thymus and hypophyseal gland

Structure	Level	Pattern	Image	Note
TS23: diaphragm	Strong		8E	Strong expression in diaphragm, pericardium, auricle and lung pleura.
TS23: heart atrium	Strong		8E	Strong expression in diaphragm, pericardium, auricle and lung pleura.
TS23: pericardium	Strong		8E	Strong expression in diaphragm, pericardium, auricle and lung pleura.
TS23: pleura	Strong		8E	Strong expression in diaphragm, pericardium, auricle and lung pleura.
TS23: liver	Absent		8E

Structure	Level	Pattern	Image	Note
TS22: renal capsule	Strong		8F
TS22: adrenal gland	Strong	Regionally restricted	8F	Strong expression capsules of liver and adrenal gland.
TS22: liver	Strong	Regionally restricted	8F	Strong expression capsules of liver and adrenal gland.
TS22: metanephros	Present	Regionally restricted	8F	Expression in kidney parenchyma.
TS22: bladder	Present	Regionally restricted	8F	Expression in bladder wall.
TS22: gut epithelium	Absent		8F	No expression in glandular epithelium of pancreas or gut epithelium.
TS22: pancreas epithelium	Absent		8F	No expression in glandular epithelium of pancreas or gut epithelium.

Structure	Level	Pattern	Image	Note
TS22: cranium	Present		8G	Expression in developing skull, ventricles and choroid plexus.
TS22: lateral ventricle choroid plexus	Present		8G	Expression in developing skull, ventricles and choroid plexus.
TS22: lateral ventricle ependyma	Present		8G	Expression in developing skull, ventricles and choroid plexus.
TS22: cerebellum	Absent		8G
TS22: cerebral cortex	Absent		8G
TS22: corpus striatum	Absent		8G
TS22: medulla oblongata	Absent		8G