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Gene Expression Data
Assay Details
Assay
Reference: J:270646 Limi S, et al., Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem. 2018 Aug 24;293(34):13176-13190
Assay type: RNA in situ
MGI Accession ID: MGI:7618431
Gene symbol: Cryaa
Gene name: crystallin, alpha A
Probe: alphaA-Crystallin exon oligo
Probe preparation: Antisense RNA
Visualized with: Fluorescence
Assay notes: Probes were detected by Quasar 570 (red).
Results
Specimen 2 region a: postnatal day 1 (more )
Note: This region represents the least differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted 2_region a alphaA Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen 2 region b: postnatal day 1 (more )
Note: This region represents the intermediate differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted 2_region b alphaA Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen 2 region c: postnatal day 1 (more )
Note: This region represents the most highly differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted 2_region c alphaA Expression is as one or two transcription sites per individual nuclei and is reduced during lens fiber differentiation and is absent in the area where denucleation has occurred.
Specimen 2 region d: postnatal day 1 (more )
Note: This region contained degraded pyknotic nuclear remnants with no intact nuclei.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted 2_region d alphaA Expression is in early differentiating lens fiber cells and is absent in the area where denucleation has occurred (shown).
Specimen 3 region a: embryonic day 12.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS20: lens primordium primary lens fiber Present Regionally restricted 3_region a alphaA Expression is in the early differentiating primary lens fibers.
Specimen 3 region b: embryonic day 12.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS20: lens primordium primary lens fiber Present Regionally restricted 3_region b alphaA Expression is in the early differentiating primary lens fibers.
Specimen 3 region c and d: embryonic day 12.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS20: lens primordium primary lens fiber Present Regionally restricted 3_region c and d alphaA Expression is in the early differentiating primary lens fibers.
Specimen 4 region a: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region a alphaA Expression is in early differentiating secondary lens fiber cells.
Specimen 4 region b: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region b alphaA Expression is in early differentiating secondary lens fiber cells.
Specimen 4 region c and d: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region c and d alphaA Expression is in early differentiating secondary lens fiber cells.
Specimen 5 region a: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region a alphaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region b: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region b alphaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region c: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region c alphaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region d: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region d alphaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 9B WT: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9B alphaA WT
Specimen 9B Snf2hnull: postnatal day 1; Smarca5tm2.1Ais/Smarca5tm2.1Ais (more )
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9B alphaA Snf2hnull
Specimen S2: postnatal day 1 (more )
Note: Double labeled: red - Cryaa mRNA; green - Cryaa mRNA (assay MGI:7618445).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted S2_alphaA intron/exon , S2_alphaA exon Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen S4 region a: postnatal day 1 (more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted S4a Merge , S4a alphaA Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen S4 region b: postnatal day 1 (more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted S4b Merge , S4b alphaA Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen S4 region c: postnatal day 1 (more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Regionally restricted S4c Merge , S4c AlphaA Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
Specimen S7: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: lens epithelium Present Punctate S7_alphaA
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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